摘要
目的观察转化生长因子-β1(transforming growth factor-β1,TGF-β1)在大鼠肝纤维化组织中的动态表达,探讨TGF-β1在肝纤维化中的意义。方法采用腹腔内注射二甲基亚硝胺(DMN)构建大鼠肝纤维化模型,造模后4天、1周、2周、4周、6周、8周分别检测血清ALT、AST、ALB的变化,同时取肝组织用半定量RT-PCR方法检测TGF-β1 mRNA的表达。采用HE染色及Masson三色染色,光学显微镜下观察肝组织损伤情况。采用单因素方差分析进行多组均数间的比较。结果肝纤维化模型组血清ALT、AST明显升高,ALB明显下降。TGF-β1 mRNA在对照组大鼠和肝纤维化模型组大鼠肝组织中均有表达。与对照组相比,肝纤维化模型组4天～1周时,TGF-β1 mRNA表达差异无统计学意义(P均>0.05)。2～4周较对照组显著升高(P均<0.05),4周时达高峰。6～8周较4周时显著下降(P均<0.05),但仍显著高于对照组(P均<0.05)。8周较6周时下降,差异无统计学意义(P>0.05)。TGF-β1 mRNA表达与肝纤维化病程呈正相关(P<0.01)。结论 TGF-β1 mRNA在正常SD大鼠肝脏中有表达,在肝纤维化大鼠肝组织中表达增加,与大鼠肝脏病理分期正相关。
Objective To observe the dynamic expression of TGF-β1 in liver fibrosis,and explore its significance.Methods SD rat models with hepatic fibrosis were established by intraperitoneal injection of dimethyl nitroxide（DMN）.Serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and albumin（ALB） levels were tested in model group at the time of 4 days,1 weeks,2 weeks,4 weeks,6 weeks and 8 weeks.TGF-β1 mRNA in hepatic tissue were detected by semiquantitative RT-PCR.Pathological characters of liver tissue were observed under optical microscope after hematoxylin-eosin and Masson staining.Comparisons between groups were analyzed by one-way ANOVA.Results ALT and AST levels in model group were significantly higher than those in control group,but ALB in model group decreased.TGF-β1 mRNA expressed in both control group and model group.Compared with control group,the expression of TGF-β1 mRNA of the model group at day 4 and week 1 had no significant difference,but were significantly higher at Week 2 to 4,and reached the peak at week 4,then decreased significantly at Week 6 to 8,which was still significantly higher than that of control group.There was no difference in the expression of TGF-β1 mRNA between 6 week group and 8 week group.The expression of TGF-β1 mRNA was positively relative with liver fibrosis.Conclusion TGF-β1 mRNA expresses in the liver of normal SD rats and expressed dynamically in rats with liver fibrosis.Its expression increases in hepatic fibrosis,and was related with pathological stages.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第2期150-152,共3页
Chinese Journal of Microecology
