摘要
采用响应面法对枸杞叶粗多糖提取中的料液比、提取温度和提取时间进行优化,确定各因素最佳的水平组合。结果显示,在料液比1:47.2,提取温度72.9℃、提取时间2.2h条件下,枸杞叶粗多糖提取物中多糖的含量最高,为(7.24±0.41)%。采用D101大孔树脂和DEAE-52阴离子交换柱对枸杞叶粗多糖进行纯化,纯化后的精多糖中多糖含量为92.5%。用α-D-吡喃葡萄糖苷(p-NPG)法对枸杞叶精多糖的α-糖苷酶抑制活性进行了测定,1mg/mL枸杞叶精多糖对5U/mL的糖苷酶抑制率达到93.7%,而阳性对照药阿卡波糖抑制率仅为21.7%。
Response surface methodology （RSM） was applied to optimize process parameters for the extraction of crude polysaccharides from Chinese wolfberry （Lycium chinense） leaves such as solid-to-solvent ratio, temperature and time. The maximum polysaccharide content of crude extracts was （7.24±0.41）% under the optimized conditions of 72.9℃, 2.2 h and a solid-to-solvent ratio of h47.2, and was increased to 92.5% after purification with D101 macroporous resin and DEAE-52 anion exchange resin column chromatography. The inhibition rate of 1 mg/mL purified polysaccharides from Chinese wolfberry leaves against 5 U/mL a-glycosidase, as determined by p-NPG method, was 93.7% compared to only 21.7% for acarbose as a positive control.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第4期42-46,共5页
Food Science
基金
"十一五"国家科技支撑计划项目(2007BAI45B00)
关键词
枸杞叶
提取工艺
多糖
Lycium chinense leaves
extraction method
polysaccharides
