摘要
目的 分析抑癌蛋白 (p5 3、p16Ink4、p2 1Waf1、p2 7Kip1)在胰腺癌组织中的异常表达及其与临床和病理特征的关系。方法 采用单克隆抗体及免疫组化Envision法分别检测了 35例胰腺癌、14例壶腹癌和 11例慢性胰腺炎组织中p5 3、p16、p2 1和p2 7的表达情况。结果 p5 3在胰腺癌和慢性胰腺炎的表达阳性率分别为 6 2 9%和 0 (P <0 0 5 )。p16、p2 1和p2 7在胰腺癌的表达缺失率分别为6 0 0 %、37 1%和 6 2 9% ,缺失率明显高于慢性胰腺炎 (P <0 0 5 )。 80 %的胰腺癌组织有 2种以上抑癌蛋白的表达异常。不同抑癌蛋白表达之间无明显相关。除p16表达与肿瘤分化程度相关 (P <0 0 5 )外 ,4种抑癌蛋白表达与年龄、性别、肿瘤大小、部位、淋巴结转移和临床分期等无明显相关。结论 胰腺癌组织中可存在多种抑癌蛋白的表达异常 。
Objective To investigate the expression of antioncogene products (p53、p16Ink4、p21Waf1 and p27Kip1) in pancreatic carcinoma(PCa) and their relationship with clinicopathological findings Methods 35 specimens of PCa,14 of ampulla carcinoma and 11 of chronic pancreatitis (CP)were obtained by surgery and p53、p16、p21 and p27 proteins were immunohistochemically stained respectively by using Envision system Results p53 protein was positively stained in 22 out of 35 PCa specimens (629%),with a rate much higher than that in CP(P<005) 20(600 %) 、13(371%) and 22(629%) of 35 PCa specimens showed loss of p16、p21 and p27, all of which except p27 showed a significant difference when compared with CP(P<005) Eighty percent of PCa specimens showed abnormality of more than 2 kinds of antioncogene products There was no significant correlation between any two proteins, neither between expression of antioncogene products and the age,gender, tumor site, tumor size, clinical stages and lymph nodes metastasis However, p16 protein expression was correlated with histological grade Conclusion Abnormal expression of antioncogene products was common in PCa, abrogation of tumorsuppressive pathways might play an important role in the carcinogenesis
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第5期315-318,I008,共5页
Chinese Journal of Internal Medicine
基金
卫生部直属医疗机构临床学科重点基金资助!(9700001)
关键词
胰腺肿瘤
免疫组织化学
抑癌蛋白
Pancreatic carcinoma
Immunohistochemistry
Anti-oncogene products
