摘要
目的:探讨肿节风对60Coγ射线照射后小型猪腮腺细胞凋亡、B细胞淋巴瘤基因-2(bcl-2)/bcl-2相关X蛋白(bax)蛋白表达的影响及其机制。方法:将60头小型猪随机分为空白组、单纯照射组、肿节风(0.3 g.kg-1)+照射组3个大组,每个大组再随机分为a,b,c,d 4个平行组。肿节风+照射组于照射前1周经口给予肿节风至腮腺取出,每日1次,其他2组给予等量生理盐水。单纯照射组和肿节风+照射组进行60Coγ射线照射,放射总剂量为30Gy,空白组不给予照射。a,b,c,d 4个平行组分别于照射后1,10,40,90 d取腮腺,采用Western blotting法检测腮腺组织bcl-2和bax的表达,TUNEL法检测腮腺细胞的凋亡。结果:在同一时点(同一平行组),bcl-2蛋白均表现为空白组>肿节风+照射组>单纯照射组;bax蛋白均表现为单纯照射组>肿节风+照射组>空白组;单纯照射组和肿节风+照射组腮腺细胞的凋亡率均较空白组增高(P<0.05)。单纯照射组中,bcl-2蛋白表达量在照射结束后第10天为最低、第90天为最高;bax蛋白在第10天为最高、第90天为最低;凋亡率在照射结束后第10天为最高(P<0.05)。肿节风+照射组中,bcl-2蛋白表达量逐渐递增;bax蛋白表达量逐渐递减;凋亡率第一天最高(P<0.05)。结论:肿节风对bax蛋白表达有抑制作用,对bcl-2蛋白表达有促进作用,可以一定程度上缓解小型猪腮腺细胞的凋亡。肿节风通过上调bcl-2、下调bax的表达可能是抑制腮腺细胞凋亡的主要机制之一。
Objective：To explore the influence and mechanism of Sarcandra Glabra on apoptosis and expression of B-cell lymphoma-2（bcl-2）/bcl-2 associated x protein（bax） of parotid glands in the miniature pig irradiated by 60Co γ rays. Method： Sixty miniature pigs were randomly divided into normal control group, radiation control group and medicine radiation group（0.3 g·kg-1）,then each group was randomly divided into parallel group numbered a,b,c and d. Sarcandra Glabra Herb was given to the corresponding groups from a week before exposure to the irradiation until the parotid glands were removed. Each pig in the radiation control group and the medicine radiation group was received 60Co gamma-rays with parotid gland（total radiation dose：30 Gy）, which in the normal control group was not received rays.On the 1th day, 10th day,40th day and 90th day after irradiation,the parotid glands of miniature pigs were removed for experiment. The expression of bcl-2/bax was detected by Western blotting,and the apoptosis was detected by TUNEL method. Result： The expression of bcl-2 in medicine radiation group was incresed compared with radiation control group on every time point. The expression of bax in three groups decreased. The apoptosis rate in the radiation control group and the medicine radiation group was higher than the normal control group on every time point（P〈0.05）. In radiation to control group, the minimum of expression of bcl-2 was in 10th day,and the maximumwas in 90th day. The highest expression of apoptosis is in 10th day,then decreased with time（P〈0.05）. Conclusion： Sarcandra Glabra diminished the expression of bax and apoptosis,while augmented the expression of bcl-2. The mechanism of Sarcandra Glabra reducing apoptosis may be related to augmenting the expression of bcl-2 and diminishing the expression of bax.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期177-181,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30960103)
关键词
肿节风
辐照
猪腮腺
bcl-2
BAX
细胞凋亡
Sarcandra Glabra
irradiation
parotid gland of miniature pig
bcl-2/bax
apoptosis
