摘要
目的观察饮水氟染毒对雄性SD大鼠曲细精管生殖细胞DNA损伤及凋亡的影响。方法将60只SPF级雄性SD大鼠随机分为4组,分别为对照(蒸馏水)组和低(10 mg/L)、中(50 mg/L)和高(100 mg/L)浓度氟化钠染毒组,每组15只。采用自由饮用方式进行染毒,连续染毒120 d。分别于染毒第60、90、120天,从每组随机抽取5只大鼠,采用硫代巴比妥酸法、单细胞凝胶电泳(SCGE)和流式细胞技术分别检测各组生殖细胞中丙二醛(MDA)含量、DNA损伤及细胞凋亡情况。结果与对照组比较,各染氟周期中、高浓度氟化钠染毒组大鼠曲细精管生殖细胞中MDA含量均明显升高(P<0.05);60和120 d时各浓度氟化钠染毒组大鼠曲细精管生殖细胞彗尾长和Olive尾矩较高(P<0.05);但90 d时各浓度氟化钠染毒组大鼠曲细精管生殖细胞彗尾长和Olive尾矩无显著变化。与对照组比较,60和90 d时高浓度氟化钠染毒组及120 d时中、高浓度氟化钠染毒组大鼠曲细精管生殖细胞凋亡率均较高(P<0.05);随着染毒时间的延长,中、高浓度氟化钠染毒组大鼠曲细精管生殖细胞凋亡率呈逐渐增高的趋势。结论过量氟通过诱导大鼠曲细精管生殖细胞脂质过氧化和DNA损伤进而激活生殖细胞凋亡,这可能是氟中毒雄性生殖损害的重要作用机制之一。
Objective To study the effects of fluoride on DNA oxidative damage and apoptosis of reproductive cells in seminiferous tubules of rats. Methods Sixty SD rats of SPF grade were randomly divided into control group and low,moderate and high-dose fluoride groups, 15 in each and treated with sodium fluoride through drinking water at the doses of 0, 10,50 and 100 mg/L for 60,90,120 days,respectively. MDA content,DNA oxidative damage and apoptosis in reproductive cells of seminiferous tubules were measured by TBA method, single cell gel electrophoresis （SCGE） and flow cytometry （FCM）, respectively. Results MDA content in reproductive cells increased significantly in moderate and high-dose groups compared with control group （P〈O.05）. However, under the same exposure dose, there was no significant difference in MDA content among all fluoride groups exposed for different time. The comet tail lengths and Olive tail moment in 60 d and 120 d fluoride subgroups increased significantly （P〈0.05）, but DNA damage in 90 d subgroup was not obvious. Compared with control group, apoptosis in high-dose group exposed for 60 d, 90 d and moderate and high-dose groups exposed for 120 d increased significantly （P〈O.05）. The apoptosis of reproductive cells in seminiferous tubules increased in an obvious dose-dependent manner. As the extension of exposure time,apoptosis of reproductive cell in moderate and high fluoride groups increased gradually. Conclusion The possible genetic toxic pathogenesis of fluoride is that excessive fluoride can induce lipid peroxidation and DNA damage in reproductive cells of seminiferous tubules which activates cell apoptosis of reproductive cells.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第2期128-131,共4页
Journal of Environment and Health
基金
广东省自然科学基金(9151022401000003)
2010年广东药学院大学生创新实验项目
关键词
氟
曲细精管
DNA损伤
细胞凋亡
Fluoride
Seminiferous tubules
DNA damage
Apoptosis
