摘要
目的:分析寿胎丸对复发性流产小鼠蜕膜蛋白质组的影响,从分子水平探讨寿胎丸安胎的作用机制。方法:50只雌性CBA/J小鼠分别与20只雄性DBA/2和5只雄性BALB/C小鼠按2∶1合笼交配,建立复发性流产模型40只与正常妊娠模型10只。将复发性流产CBA/J×DBA/2孕鼠按妊娠先后顺序随机分为模型组(10只)、寿胎丸低剂量组(10只,3 g.kg.d-1)、寿胎丸中剂量组(10只,6 g.kg.d-1)和寿胎丸高剂量组(10只,12 g.kg.d-1),从妊娠第1天开始灌胃给药,至孕14 d处死小鼠,计算各组胚胎丢失率。采用双向电泳结合质谱技术分离和鉴定各组小鼠蜕膜差异表达的蛋白质,并利用生物信息学资源对所鉴定蛋白质的功能进行分析。结果:寿胎丸高、中剂量组胚胎丢失率较模型组均明显降低(P<0.01),寿胎丸低剂量组胚胎丢失率有所降低,但与模型组相比差异无统计学意义;建立了模型组、正常组、寿胎丸高、中、低剂量组小鼠蜕膜组织的2-DE图谱,通过比较各组双向凝胶电泳图谱的差异,发现并鉴定了30个可能与复发性流产及寿胎丸干预相关的蛋白质,这些蛋白质的功能涉及绒毛外滋养细胞的侵袭、蜕膜组织血管的重建及蜕膜细胞的凋亡等。结论:寿胎丸能够降低复发性流产小鼠胚胎丢失率,具有显著的安胎作用;复发性流产是由多种蛋白质参与的复杂过程,寿胎丸可调节复发性流产小鼠蜕膜组织多种蛋白质的表达,该复方具有多靶点的作用特点。
Objective： To analysis the differential expression of deeidua tissue proteins and effective mechanisms of recurrent abortion mice with Shoutai Wan, and explore the mechanism of Shoutai Wan in preventing miscarriage. Method ： The abortion-prone CBA/J x DBA/2 matings were established as the model of recurrent abortion and the nonabortion-prone CBA/J x BALB/c matings were used as the model of normal pregnancy. The model of recurrent abortion CBA/J × DBA/2 of mice pregnant were randomly divided into four groups according to the sequence of pregnancy, including model group, Shoutai Wan low-dose group, Shoutai Wan middle-dose group and Shoutai Wan high-dose group. From the 1st day of pregnant, mice of normal group, model group, Shontai Wan low-dose group （3 g ·kg · d-1 ） ,Shoutai Wan middle-dose group（6 g · kg· d-1 ） and Shontai Wan high-dose group（ 12 g · kg ·d-1 ） are oral administration in different doses. On the 14th day of pregnancy,all mice are killed and the embryo loss rate（ELR） was counted. The expression of differential proteins of mice decidua tissues were separated by means of 2-DE and identified by MALDI-TOF-MS. The functions of identified proteins were further analysed according to bioinformatics resources. Result： Compared with model group, low-dose Shoutai Wan can not significantly improve the model of recurrent abortion in pregnant mice ELR;Shoutai Wan middle-dose and highdose group of pregnant mice ELR were significantly decreased （P 〈 0. 01 ）. The results showed that the well-resolved, reproducible 2-DE patterns of mice decidua tissues of model group, normal group and Shoutai Wan low middle high-dose group were obtained. Through comparative proteome analysis of deeidua tissues of all groups,30 differential expression protein spots which maybe related to recurrent abortion and Shoutai Wan intervention were identified by MALDI-TOF-MS. These differential expression proteins mainly refer to invasion of the blastocyst, blood vessel remodeling and cell apoptosis. Conclusion： Shoutai Wan can decrease recurrent abortion mice ELR significantly,and play a role in preventing miscarriage. Recurrent abortion is a complicated process refer to diverse proteins partici- pate. For several protein spots expression of decidua tissues in recurrent abortion mice was regulated by Shoutai Wan,it provides contribution to the effect characteristic of multitarget.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第4期591-596,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30973771)
教育部博士点基金博士导师项目(20094323110004)
