摘要
采用激光镊子拉曼光谱(LTRS)技术对圆红冬孢酵母合成油脂和类胡萝卜素进行定量分析,考察不同碳氮比、碳磷比以及碳硫比对圆红冬孢酵母在细胞水平油脂和类胡萝卜合成能力的影响。测定不同浓度植物油的拉曼光谱,其1751cm-1峰峰高与油脂浓度线性关系良好,相关系数R2可达0.97349,说明激光镊子拉曼光谱法是定量分析油脂的有效手段。以1751cm-1峰和1520cm-1峰峰高定量细胞内油脂和胡萝卜素含量,分析不同样品圆红冬孢酵母细胞的拉曼光谱,结果表明,随着碳氮比、碳磷比和碳硫比的升高,圆红冬孢酵母细胞内油脂含量逐步增加,而类胡萝卜素的含量则显著降低。以上结果说明,激光镊子拉曼光谱技术是实时检测圆红冬孢酵母细胞油脂及类胡萝卜素合成和优化发酵培养基的有效工具。
A method is established based on laser tweezer and carotenoids in Rhodosporidium toruloides cells, and Raman spectroscopy （LTRS） for rapid quantitation of lipid the effect of different C/N, C/P, C/S ratios in a culture medium on synthesis capability of lipid and carotenoids is evaluated at the single-cell level. The Raman spectra of plant oil in different concentrations are determined and the high correlation between the peak intensity at 1751 cm^-1 and lipid concentration is observed. The correlation coefficient of standard curve for lipid is 0. 97349. The data demonstrate that LTRS is efficient in the quantitative analysis of lipid. Lipid and carotenoids within the Rhodosporidium toruloides cells cultured in different media are acquired quantificationally according to the Raman peak heights at 1751 cm^-1 and 1520 cm^-1, respectively. The result shows that the lipid concentration in Rhodosporidium toruloides cells increases as the ratios of C/N, C/P, C/S increase, while the carotenoids concentration significantly decreases. The above results indicate that LTRS is an efficient approach to the quantitative analysis of lipid and carotenoids in microbial cells and the optimization of fermentation medium.
出处
《激光与光电子学进展》
CSCD
北大核心
2013年第3期174-179,共6页
Laser & Optoelectronics Progress
基金
国家自然科学基金(31060128)
广西科学院基本科研业务费(11YJ24WL01)资助课题
关键词
光谱学
定量检测
激光镊子拉曼光谱
圆红冬孢酵母
油脂
类胡萝卜素
spectroscopy
quautitative determination
laser tweezer Raman spectroscopy
Rhodosporidium toruloides
lipid
carotenoids
