摘要
目的 观察激素对骨髓基质细胞脂肪特异性基因aP2mRNA及成骨基因Ⅰ型胶原mRNA表达的影响。方法 以地塞米松 (1× 10 -7mol/L)作为诱导剂 ,采用完整细胞斑点印迹分子杂交方法检测实验组和对照组中aP2和Ⅰ型胶原mRNA表达。结果 实验组aP2mRNA含量4847.7± 40 6 .4明显高于对照组 15 7.6± 10 .8,其Ⅰ型胶原mRNA含量 44 2 .3± 5 7.8明显低于对照组 5 35 3 .6± 36 4.6。结论 地塞米松能够从基因调控水平诱导骨髓基质细胞向脂肪细胞分化 ,减少其向成骨细胞分化 ,这可能与激素性骨坏死的发病机制有关。
Objective To observe the effect of steroids on the expression of anadipose specific gene, aP2 mRNA, and osteoblastic gene, collagen type I mRNA, of marrow stromal cells.Methods The expression level of aP2 and collagen type I mRNA in the experimental groups with the treatment of 1×10 -7 mol/L dexamethasone and the controls was detected by intact cell RNA dot blots. Results The aP2 mRNA contents in the experimental group 4 847.7±406.4 were obviously higher than in the control group 157.6±10.8, whereas the collagen type I mRNA contents in the experimental group 442.3±57.8 were significantly lower than in the control group 5 353.6± 364.6.Conclusion Dexamethasone could induce marrow stromal cell differentiation into adipocytes and inhibit osteoblastic differentiation by regulating gene expression, which might responsible for the development of the steroid induced osteonecrosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第6期557-558,共2页
Chinese Journal of Experimental Surgery
基金
河南省科委自然科学基金!资助项目 (9840 2 170 0 )
关键词
骨坏死
地塞米松
骨髓基质细胞成脂
成骨分化
Glucocorticords
Bone marrow
Cell differentiation
Gene expression
Osteonecrosis
