摘要
目的观察肠易激综合征(IBS)模型WHBE兔神经内分泌激素和电解质水平的变化,探讨神经-体液-内分泌系统在IBS中的作用。方法以湿热环境应激加小剂量致泻剂诱导WHBE兔建立IBS模型,在造模9 d和14 d时取血分离血清,检测血清中5-羟色胺(5-HT)、多巴胺(DA)、促肾上腺皮质激素(ACTH)、皮质醇(COR)、β-内啡肽(β-EP)激素和K+、Na+、Cl-含量的变化,并以JW兔作对照。结果与正常对照组比,造模9 d时WHBE兔的5-HT、DA、ACTH、β-EP、COR均显著升高(P<0.05,P<0.01),Na+、K+含量均显著降低(P<0.01),而JW兔ACTH、β-EP、COR均显著升高(P<0.05,P<0.01),K+含量显著降低(P<0.05);造模14 d时WHBE兔5-HT、ACTH、β-EP均显著升高(P<0.05,P<0.01),JW兔ACTH显著升高(P<0.05);相关分析显示,IBS模型中血清中5-HT、COR与Na+、K+浓度呈显著负相关(P<0.05,P<0.01),血清中DA、β-EP水平与Na+、K+、Cl-浓度呈显著负相关(P<0.05,P<0.01),ACTH水平则与K+浓度呈显著负相关(P<0.01)。结论 IBS的发生不仅与HPA轴兴奋性升高有关,亦与体液因素有关,故神经-体液-内分泌系统参与了WHBE兔IBS的发生过程。
Objective To observe the changes of neuroendocrine hormone and electrolyte levels in WHBE rabbit with irritable bowel syndrome, and to explore the regulatory role of neurohumoral-endocrine system. Methods The rabbits model of irritable bowel syndrome was induced by moist heat, stress and stimulation with low-dose cathartic. Blood samples were taken at 9 d and 14 d of modeling, and the changes of serum dopamine （DA）, 5-hydroxytryptamine （5- HT）, adrenocorticotropie hormone （ACTH）, cortisol （COR）, β-endorphin （β-EP） hormone and K^＋ , Na^＋ , C1^- content were determined and compared with those of the JW rabbits. Results Compared with the normal control group, the DA,5-HT, ACTH, β-EP and COR contents were significantly increased （P 〈 0. 05, P 〈 0. 01 ） , and the Na^＋ , K^＋ contents were significantly lower （P 〈 0. 01 ） in the WHBE rabbit models at 9 days of modeling; and the ACTH, β-EP and cortisnl levels were significantly increased （ P 〈 0.05, P 〈 0. 01 ） , and K ^＋ content was significantly decreased （ P 〈 0.05 ） in the JW rabbits at 9 days of modeling. At 14 days of modeling, compared with the control group, the serum 5-HT, ACTH, β-EP were significantly increased （P 〈 0. 05, P 〈 0. 01 ） in the WHBE rabbit group, and the serum ACTH was significantly increased （P 〈 0. 05 ） in the JW rabbit group. The correlation analysis showed that in the IBS models there was a significant negative correlation between the serum 5-HT, COR and Na^＋ , K＋ concentrations （ P 〈 0. 05, P 〈 0. 01 ）, between the serum DA, β-EP levels and Na^＋ , K^＋ , C1- concentrations （P 〈 0.05, P 〈 0. 01 ）, and between the ACTH levels and K^＋ concentration （ P 〈 0. 01 ）. Conclusion Irritable bowel syndrome is not only associated with elevated excitability of the hippothalamus-pituitary-adrenal axis, but also related with humoral factors. Therefore, neurohumoral- endocrine system is involved in the pathohenesis of irritable bowel syndrome in WHBE rabbits.
出处
《中国比较医学杂志》
CAS
2013年第2期26-31,共6页
Chinese Journal of Comparative Medicine
基金
浙江省教育厅重大科技攻关项目(ZD2009004)
"浙江省卫生高层次创新人才培养工程项目"资助
