摘要
目的:观察四磨汤对不同胃肠功能障碍模型小鼠胃肠运动及血清胃动素(MTL)、生长抑素(SS)的影响。方法:将140只小鼠随机分为正常组(20只)、慢性应激组(40只)、脾虚便秘组(40只)和燥结便秘组(40只);造模成功后除正常组外其余3组随机分为相应的模型组和治疗组各20只;各治疗组给予四磨汤口服液灌胃7d后,进行胃排空和小肠推进试验,Elisa法测定血清中MTL、SS含量。结果:不同模型组小鼠的胃内残留率升高,小肠推进率降低,血清MTL含量降低,慢性应激模型组和脾虚便秘模型组SS含量升高,与正常组比较差异均有统计学意义(P<0.05,P<0.01)。用药后慢性应激治疗组胃内残留率降低,小肠推进率升高,血清MTL水平上升、SS水平下降,与慢性应激模型组比较差异有统计学意义(P<0.05);上述指标在脾虚便秘治疗组与脾虚便秘模型组、燥结便秘治疗组与燥结便秘模型组之间差异不显著。结论:四磨汤对慢性应激模型小鼠作用明显,可能与调节胃肠运动功能及调节脑肠肽的水平变化有关。
Objective： To observe the effect of Simo Decoction on gastrointestinal motility and MTL, SS in serum of different mice models of gastrointestinal dysfunction. Methods： 140 mice were randomly divided into normal group（20）, chronic stress group（40）, spleen deficiency constipation group（40） and fluid-consuming constipation group（40）. Except the normal group, other three groups were divided into the corresponding model and treatment group of 20 after the success of modeling, and then treated with Simo Decoction for 7 days, tested for function of gastric emptying and small intestinal propulsion. The contents of MTL and SS were tested by Elisa. Results： The gastric residual rate increased, small intestinal propulsion rate and contents of MTL depressed. The contents of SS increased in model groups with chronic stress and spleen deficiency constipation. All of them had statistically significant difference compared with normal group（P〈0.05, P〈0.01）. After treatment, the gastric residual rate and SS level depressed, small intestinal propulsion rate and MTL level increased in treatment group with chronic stress（P〈0.05）. The difference of above-mentioned index in spleen deficiency constipation groups and fluid-consuming constipation groups was not significant. Conclusion： Simo Decoction has obvious effect on chronic stress model mice, which may be related to regulation of gastrointestinal motor function and brain-gut peptide levels.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第3期772-774,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家重点基础研究计划(973计划)(No.2009CB523002)
湖南省中医药科技计划项目(No.2009004)~~
关键词
四磨汤
胃排空
小肠推进
胃动素
生长抑素
功能性便秘
慢性应激
Simo Decoction
Gastric emptying
Small intestinal propulsion
Motilin
Somatostatin
Functional constipation
Chronic stress
