摘要
目的:运用代谢组学方法研究乙醇致胃黏膜损伤大鼠机体代谢表型的变化及黄连总生物碱发挥保护作用的相关代谢途径。方法:利用核磁共振氢谱(1H-NMR)技术建立大鼠血清和尿液的代谢指纹谱。应用正交偏最小二乘判别分析研究对照组和模型组之间的代谢物谱差异,并通过变量重要性投影(VIP)在血清和尿液中选取生物标志物。使用主成分分析法研究黄连总生物碱对乙醇致大鼠胃黏膜损伤的保护作用。通过比较选取的生物标志物的含量变化,探讨黄连总生物碱发挥保护作用的途径。结果:乙醇致胃黏膜损伤大鼠血清和尿液代谢表型发生了明显的变化,在血清和尿液中分别鉴定了与胃黏膜损伤相关的8种和10种生物标志物。结论:胃黏膜损伤大鼠机体糖代谢、脂质代谢和氨基酸代谢紊乱,同时,肠道菌群受到一定程度的扰动。黄连总生物碱能有效地缓解胃黏膜损伤大鼠机体脂质和糖代谢失衡,同时,可以调节肠道菌群。运用代谢组学技术可以从整体上揭示黄连总生物碱对乙醇致大鼠胃黏膜损伤的保护作用与机制。
Objective： To investigate the metabolic profile of alcohol-induced gastric lesion in rats and the protective effects of total alkaloids from Coptis chinensis. Methods： Proton nuclear magnetic resonance（1H-NMR） technique was used to analyze the rat metabolin. Orthogonal partial least squares discriminant analysis（OPLS-DA） was processed to analyze the metabolin difference between the control and alcohol treated samples. Potential biomarkers were selected in rat serum and urine via the parameter of variable importance in the projection（VIP）. Principal components analysis（PCA） was employed to process the data from the total alkaloids treatment group and the protective effect of total alkaloids was investigated through the selected potential biomarkers. Results： After alcohol treatment, the metabolic phenotype of rats greatly changed. Eight and ten potential biomarkers were selected in rat serum and urine, respectively. Conclusion： After alcohol treatment, the lipids metabolism, glucose metabolism, amino acids metabolism and gut microflora environment were seriously disturbed. The total alkaloids from Coptis chinensis could effectively alleviate the disturbance of lipids and glucose metabolism and modulate the gut microflora environment. The therapeutic mechanism of total alkaloids from Coptis chinensis on alcohol-induced gastric lesion in rats was revealed integrally by metabonomics in this study.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第3期807-810,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.81050019)
广东高校优秀青年创新人才培养计划项目(No.粤财教[2009]400)
广东省科技计划项目(No.2012B060300031)~~
关键词
代谢组学
核磁共振
胃黏膜损伤
乙醇
黄连总生物碱
Metabonomics
NMR
Gastric lesion
Alcohol
Total alkaloids from Coptis chinensis
