摘要
目的 探讨自体静脉移植后转化生长因子β1(TGFβ1)和血管内皮细胞生长因子 (VEGF)表达的动态变化及其与内膜增生的关系。 方法 10 0只Wistar大鼠 ,分为 10组 ,第 1组为对照组 ,第 2～ 9组为自体静脉移植模型组。应用原位杂交和免疫组化方法 ,观察大鼠自体移植静脉后不同时期 ,移植静脉的内膜增生情况、TGFβ1mRNA、TGFβ1和VEGF蛋白表达的变化动态。 结果 自体静脉移植后 2周 ,移植静脉内膜明显增生 ,厚度达 (4 4 44± 3 5 0 ) μm ;8周时内膜增生达高峰 ,厚度达(12 4 6 8± 14 30 ) μm。TGFβ1mRNA表达在静脉移植术后 1周达 46 7% ,以后逐渐下降 ,但术后 10周时 ,其阳性细胞仍达 10 1%。TGFβ1和VEGF蛋白表达均在移植 2 4h后开始增加 ,术后 2周达到高峰 ,其阳性细胞分别为 40 6 %和 36 6 % ;4周以后两者表达均下降 ,至 8周时阳性细胞分别为 13 8%和8 9%。 结论 自体静脉移植后 ,TGFβ1可能通过促进细胞外基质 (ECM)合成和降低ECM降解促进内膜增生 ,而VEGF在静脉移植后的再内皮化过程中起重要作用 ,二者可能相互作用 ,协同参与移植静脉的内膜增生。
Objective To investigate time course of TGFβ 1 and VEGF expression and their role in intimal hyperplasia. [WT5”HZ]Methods[WT5”BZ] In situ hybridization and immunohistochemical technique were used to detect the time course of intimal hyperplasia, time course of TGFβ 1 mRNA and protein expression of TGFβ 1 and VEGF. [WT5”HZ]Results[WT5”BZ]After autogenous vein replacement, the obvious neointima was seen at 2 weeks, and peaked at 8 weeks. The expression of TGFβ 1 mRNA peaked at 1 week and decreased gradually, but at 10 weeks, its positive cell percentage was still 10 1%. Both protein expression of TGFβ 1 and VEGF in VSMCs increased from 24 hours after grafting and peaked at 2 weeks. Their positive cell percentages were 40 6% and 36 6% respectively. After 4 weeks, their expression decreased at 8 weeks, the positive cell percentages were 8 9% and 13 8% respectively. [WT5”HZ]Conclusions[WT5”BZ] TGFβ 1 plays an important part in ECM accumulation by promoting ECM synthelization and decreasing ECM degradation. VEGF plays the key role in reendothelialization. They may affect each other and cooperated in the formation of intimal hyperplasia.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2000年第11期861-864,I048,共5页
Chinese Journal of Surgery
关键词
血管内膜增生
自体静脉移植
TGFΒ1
VEGF
Transplantation, autologous
Transforming growth factor beta
Veins
Endothelial growth factors
Tunica intima
HyperplasP
