摘要
目的:研究实验性大鼠牙移动过程中,加力不同时间及力值后,香草酸瞬时亚型1(transient receptor potential vanilloid-1,TRPV1)和白细胞介素1α(interleukin-1α,IL-1α)在牙周膜中的表达规律,进而探讨两者在正畸疼痛中的作用。方法:将66只SD大鼠随机分为对照组、假手术组、实验组共11组,每组6只。实验组大鼠施50 g力后,分别于4 h、8 h、1 d(1 d组据力值大小增加30、50、80 g 3个亚族)、3 d、5 d、7 d、14 d,随机处死6只,切取牙周膜组织,采用实时定量PCR技术观察各组大鼠牙周膜内TRPV1和IL-1αmRNA的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:同一力值,牙周膜内TRPV1和IL-1α在加力4 h后升高,1 d时到达峰值,7 d后下降至正常水平。牙周膜内TRPV1和IL-1α的表达随力值增加而升高。结论:在生物机械力的诱导下,牙周膜内TRPV1和IL-1α的表达与加力时间及大小呈现规律性变化,提示TRPV1和IL-1α与正畸疼痛存在一定联系。
PURPOSE： To investigate the expression of TRPV1 and IL-lα in Sprague-Dawley rats＇periodontal ligament （PLD） during experimental tooth movement of different orthodontic force and duration, and explore the role of TRPV1 and IL-1α in orthodontic pain. METHODS： 66 Sprague-Dawley rats were divided into control group （n=6）,sham operation group （n=6） and experimental group （n=54） randomly. Orthodontic force was applied on right first maxillary molar in rats and the changes of TRPV1 and IL-lot expression were detected by real-time PCR at 4 h, 8 h, 1 d （three subgroups were added according to different forces： 1 d-30 g, 1 d-50 g, 1 d-80 g ）, 3 d, 5 d, 7 d, 14 d after tooth movement. The data was analyzed using SPSS 13.0 software pakage. RESULTS： Following the experimental tooth movement, the expression of TRPV1 and lL-lα in periodontal tissues were significantly up-regulated from 4 h to 7 d, with a peak at 1 d and returned to normal level at 1 week. The greater force applied during experimental tooth movement at 1 d, the higher expression of TRPV1 and IL-lα were detected in periodontal tissues. CONCLUSIONS： Experimental tooth movement leads to regular change of TRPV1 and IL-lαexpression in periodontal tissues, which indicates that TRPV1 and IL-lα may play an important role in orthodontic pain. Supported by National Natural Science Foundation of China （30973359） and Natural Science Foundation of Shanxi Province （2009JQ4007）.
出处
《上海口腔医学》
CAS
CSCD
北大核心
2013年第1期1-6,共6页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30973359)
陕西省自然科学基金(2009JQ4007)~~
