摘要
从珠三角某鸽场病死鸽中无菌操作采取心血、肝脏、脑、脾脏,接种于麦氏培养基和木糖—赖氨酸—脱氧胆酸琼脂培养基,经细菌纯化、镜检、生化试验、PCR检测和药敏试验,结果显示30只病鸽有8只分离到沙门氏菌,阳性率为26.7%;10只分离到大肠杆菌,阳性率为33.3%;其中6只同时分离到沙门氏菌和大肠杆菌,混合感染率为20.0%。经病毒分离培养、PCR检测,结果显示从6个混合样品中分离到5株副黏病毒,分离率为83.3%。结果表明,该鸽场鸽群发生了副黏病毒、沙门氏菌、大肠杆菌混合感染。本试验对珠三角鸽场近年较常发生的、以乳鸽较严重发病死亡的典型疫情作了较明确的诊断。
It was isolated from blood, liver, brain, spleen of mortality pigeons in a pigeon farm in the pearl river delta re- gion, inoculated on MacConkey medium and xylose, lysine, deoxychoiate medium, after bacterial purification, microscopic ex- amination, biochemical test, PCR test and drug sensitivity test, results showed that, 8 strains of Salmonella were isolated from 30 samples, the isolation rate was 26.7%, 10 strains of E. coli were isolated, the rate was 33.3%, 6 pigeons were infec ted Salmonella and E. coli, mixed infection rate was 20.0%. By virus isolation and PCR testing, results showed that 5 avian paramyxovirus of the 6 samples were isolated, the isolation rate of 83.3%. The incidence of death was caused by mixed infection of pigeon paramyxovirus, Salmonella and E. coli.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第2期155-160,共6页
China Animal Husbandry & Veterinary Medicine
