摘要
在单因素试验的基础上,采用三因素三水平响应面分析法,利用软件Box-Behnken实验设计原理,获得二次线性回归方程式,以黄酮产率为响应值作响应面图,确定微波辅助提取绿茶黄酮的最优工艺条件为:功率600 W、时间3.5 min、液固比50∶1(mL/g)、提取次数2次,黄酮产率为2.605 mg/g,验证实验表明,所得模型方程能较好地预测实验结果,拟合度较好。
Response surface methodology with three factors and three levels was used to optimize the microwave-assisted extraction of flavonoids of green tea, on the basis of a series of one-factor experiments, second linear regression equation was obtained with flavonoids yield as a response surface value by the Desigexpert software, the optimum extraction conditions of flavonoids of green tea were determined as follows : 600 W of extraction power, 3.5 min of extraction time, and 50 : 1 (mL/g) of liquid-to-solid ratio, and twice of extraction times, the extraction efficiency of flavonoids was up to 2.605 mg/g under the optimized conditions. Confirmative test indicated that the established model had good prediction ability.
出处
《食品研究与开发》
CAS
北大核心
2013年第3期19-23,共5页
Food Research and Development
基金
山西省归国留学人员科研基金(2008-90)
山西省教育厅国际交流项目(晋教外[2011]63号)
忻州师范学院院级基金(201109)
关键词
响应面法
绿茶黄酮
微波提取
response surface methodology, flavonoids of green tea
microwave-assisted extraction