摘要
目的探讨快速有效的阴道毛滴虫染色方法,以满足实验教学需求。方法将pH 6.4～6.8肝浸汤培养基转种2～3次的无菌阴道毛滴虫,在春秋季25℃和冬季10℃室温条件下,用体积分数为2%、5%、8%的Giemsa染液分别染色15、20、25、30、35、40 min,观察染色情况。结果体积分数5%的Giemsa染液在春秋季室温25℃染色20 min、冬季室温10℃染色25 min时,阴道毛滴虫外形清晰呈梨形,细胞核呈紫红色,细胞质呈浅蓝色,波动膜、鞭毛和轴柱均可以观察到。结论采用体积分数5%、pH 6.4～6.8的Giemsa染液,在室温25℃时染色20 min或室温10℃染色25 min染出的阴道毛滴虫标本比较理想,符合教学需求。
Objective To explore more fast and effective Trichomonas vaginalis dyeing method for experimental teach- ing. Methods Taking the Trichomonas vaginalis which had already inoculation for 2 - 3 times in liver soup medium with pH value for 6.4 - 6.8 ,dying under 25℃ room temperature in the spring/autumn and 10℃ room temperature in the winter re- spectively,by a concentration of 2% ,5% and 8% Giemsa dyeing solution,and the dyeing time were 15,20,25,30,35 and 40 minutes respectively. The staining state was observed. Results The appearance of Trichomonas vaginalis was cleared pear- shaped, purple cell nucleus and pale blue cytoplasm when dyeing 20 minutes in spring/autumn under 25 ~C room temperature and 25 minutes in winter under 10 ~C room temperature by volume ratio 5% Giemsa dyeing solution. The undulating mem- brane, mastigont and axon cylinder can be observed. Conclusion The dyeing result of Trichomonas vaginalis specimen is ideal with the pH value 6.4 -6.8, volume ratio 5% Giemsa dyeing solution dyeing 20 minutes in spring/autumn under 25℃, and dyeing 25 minutes in winter under 10 ~C. The specimen meet the teaching demand.
出处
《新乡医学院学报》
CAS
2013年第2期96-97,100,共3页
Journal of Xinxiang Medical University
基金
河南省教育厅自然科学研究项目(编号:2003310120)
关键词
阴道毛滴虫
染色
温度
染液浓度
Trichomonas vaginalis
dyeing
temperature
dye solution concentration
