摘要
目的研究胃肠间质瘤危险性分级与磷脂酰肌醇3激酶(PI3K)/蛋白激酶(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)信号传导通路中mTOR和磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)表达的关系。方法用免疫组织化学法检测40例胃肠间质瘤中PI3K/Akt/mTOR信号传导通路相关蛋白mTOR和p-mTOR的阳性表达率,分析其在不同危险程度组之间的差异。结果 mTOR、p-mTOR蛋白在部分胃肠间质瘤呈阳性表达,定位于细胞质。mTOR、p-mTOR在极低和低度危险组的阳性表达率均低于中高度危险组,差异均有统计学意义(P<0.05)。结论胃肠间质瘤PI3K/Akt/mTOR信号传导通路中mTOR和p-mTOR与其侵袭危险性有关,提示该信号传导通路在介导胃肠间质瘤发生、发展过程中起重要作用。
Objective To explore the relationship between the risk grade of gastrointestinal stromal tumor（GIST） and the expression of mammalian target of rapamycin （ mTOR ） and phosphorylated mammalian target of rapamycin （ p-mTOR ） in signal transduction pathway of phosphatidylinositol 3-hydroxy kinase/protein kinase B/mTOR （PI3K/Akt/mTOR）. Methods The positive expression rates of PI3K/Akt/mTOR signal transduction pathway associated protein of mTOR, p-mTOR in 40 patients with GIST was detected by the immunohistochemistry staining. Results The mTOR and p-mTOR were positive ex- pression in some GIST patients and them were localized in the cytoplasm. The positive expression rates of mTOR and p-mTOR in very low-risk and low-risk group were significantly lower than those in intermediate-risk and high-risk group （ P 〈 0.05 ）. Conclusion There expression of PI3K/Akt/mTOR signal transduction pathway associated protein of mTOR, p-mTOR is relat- ed to risk grade. It suggested that this signal transduction pathway may play an important role in the development process in GIST.
出处
《新乡医学院学报》
CAS
2013年第2期98-100,共3页
Journal of Xinxiang Medical University
基金
湖北省教育厅科学研究计划项目(编号:2009-381)
湖北省卫生厅科研项目(编号:2009JX4C15)
