摘要
[目的]建立‘幻想’矮牵牛的高效遗传转化受体再生体系。[方法]以‘幻想’矮牵牛嫩叶片为外植体,对其暗培养时间、出芽和生根培养基的生长素种类及浓度、筛选抗生素和抑菌抗生素进行优化。[结果]诱导叶片分化的最佳暗培养时间为2 d;最佳出芽培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L;最佳生根培养基为MS+IBA 0.1 mg/L;适宜出芽和生根阶段的卡那霉素筛选压为15 mg/L;适宜的抑菌抗生素头孢霉素浓度为300 mg/L。[结论]该研究为后期‘幻想’矮牵牛的分子及育种研究奠定了基础。
[ Objective ] The paper was to establish efficient regeneration system for genetic transformation of Petunia hybrida ＇ Fantasy＇. [ Method] The effects of dark incubation time, auxin types and concentrations of sprouting medium and rooting medium, screening antibiotics and antibacterial antibiotics on shoot regeneration and rooting were studied by using young leaves of P. hybrida ＇ Fantasy＇ as explants. [ Re- suit] The lrest dark incubation time for the induction of leaf differentiation was 2 days; the best medium for sprouting was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 ms/L; the best rooting medium was MS ＋ IBA 0.1 ms/L; the appropriate kanamycin screening pressure in the sprouting and rooting stage was 15 ms/L; the suitable concentration of antibacterial antibiotics cefotaxime was 300 mg/L. [ Conclusion] The study laid the foundation for the late molecular and breedim, research of P. hvbrida ＇Fantasv＇.
出处
《安徽农业科学》
CAS
2013年第4期1462-1463,1503,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金资助项目(30972020)
关键词
'幻想’矮牵牛
遗传转化
再生体系
暗培养时间
生长素
抗生素
Petunia hybrida ＇Fantasy＂
Genetic transformation
Regeneration system
Dark incubation time
Auxin
Antibiotics
