摘要
以玉米幼胚作外植体诱导愈伤组织 ,研究了幼胚长度、培养基、基因型对愈伤组织诱导和继代的影响 .结果表明 ,基因型是限制幼胚培养的主导因素 ;幼胚长度以 1 0～ 2 5mm为宜 ;试验采用的 6种培养基对出愈率影响不大 ,但它们诱导出的愈伤组织类型不同 ,在继代过程中 。
A study was carried out on the influences of the genotype, immature embryo length of maize and culture medium on the callus induction and culture by using maize immature embryo as experimental material. The results showed genotype was the main factor affecting immature embryo culture, and the immature embryo 1.0～2.5 mm in length was beneficial to the culture of calli; although six kinds of culture media used in the experiment had no difference in the frequency of producing callus from the immature embryo, they induced different types of callus, Pro and CH were beneficial to the formation of Type Ⅱ callus in the course of long term culture.
出处
《河南农业大学学报》
CAS
CSCD
2000年第4期305-308,共4页
Journal of Henan Agricultural University
基金
河南省科技攻关项目! (99116 0 10 3)
