摘要
液体深层发酵所得的羊肚菌 (MorchellaessulentaL .)营养液经超过滤法去掉小分子量组分 ,脱脂 ,脱色 ,脱蛋白质 ,减压低温浓缩 ,用超速离心法分离提纯 ,再用乙醇沉淀法反复多次将所要的多糖沉淀提纯 ,得到羊肚菌多糖 (MEP)。对其中分子量为 10 0 0 0～ 10 0 0 0 0的多糖 (MEP SP) ,采用DEAE 纤维素离子交换柱 ,以不同浓度NaCl为洗脱液 ,进行纯化分离 ,再经SepharoseCL 6B纯化得到 2种水溶性多糖MEP SP2和MEP SP3。用高效液相色谱法测得MEP SP2和MEP SP3的分子量分别为 2 30 0 0和 44 0 0 0。气相色谱法对MEP SP2和MEP SP3组成的分析结果表明 ,MEP SP2由甘露糖、葡萄糖、阿拉伯糖和半乳糖残基为重复单元组成 ,摩尔比为 1.75∶4.13∶0 .71∶0 .6 8;MEP SP3由木糖、葡萄糖、甘露糖、果糖、阿拉伯糖和半乳糖残基为重复单元组成 ,摩尔比为 3.5 8∶14.9∶3.85∶1.77∶5 1.3∶0 .5 3。用核磁共振及红外光谱法等鉴定出MEP SP2是具有α
The nutrient liquid of \%Morchella esculenta \%L. from liquid deep layer fermentation was treated by ultrafiltration to remove low molecular weight components, then defatted, decolorized and deproteinized and followed by concentration under reduced pressure and low temperature. It was further purified by ultracentrifuge and repeated precipitation and purification with ethanol to obtain the required polysaccharide components (MEP), in which MEP\|SP of molecular of 10 000～100 000 was purified and separated by DEAE\|cellulose ion exchange column and Sepharose CL\|6B gel chromatography, two soluble fractions (MEP\|SP2 and MEP\|SP3) were yielded. HPLC was used to obtain their molecular weight and gas chromatography was used to determine their monosaccharide constitution. The results showed that molecular weight of ME\|SP2 is 23 000, MEP\|SP3 is 44 000, the former is composed of four kinds of monosaccharides: mannose, glucose, arabinose and galactose in the mole ratio of 1.75∶4.13∶0.71∶0.68; the latter is composed of six kinds of monosaccharides, which contains xylose, glucose, mannose, fructose, arabinose and galactose in mole ratio of 3.58∶14.9∶3.85∶1.77∶51.3∶0.53. The structure of MEP\|SP2 was studied by means of elemental analysis, IR, NMR, etc. the result showed that main chains in MEP\|SP2 were composed of α\|pyranglycoside linkage.
出处
《植物资源与环境学报》
CAS
CSCD
2000年第2期14-17,共4页
Journal of Plant Resources and Environment
基金
北京市科研院所科技开发基金资助项目!合同编号 :95 185 12 0 0
关键词
羊肚菌
多糖
分离
纯化
结构
Morchella essulenta L.
polysaccharide
isolation
purification
characterization
