摘要
目的 通过对胰岛素受体及受体后传导介质的测定 ,寻找 2型糖尿病患者胰岛素抵抗信号传导通路中的一些共有的缺陷 ,为揭示其病因、寻找有效的治疗药物奠定基础。方法 用同位素示踪的方法 ,来测定患者外周血淋巴细胞TPK和 MAPK的活性 ;用 Fluo- 3为荧光探针 ,来测定胞浆内 Ca2 +浓度 ;分光光度法测定一氧化氮 (NO)浓度。结果 2型糖尿病病人 TPK、MAPK水平下降 ,NO浓度降低 ,Ca2 +浓度升高。结论 2型糖尿病患者胰岛素信号传导通路中有关信号分子存在缺陷。
Objective Through the determination of the insulin receptor and its signal transducers,we tried to find the defects in the common pathway of insulin signal transduction in type 2 DM and to lay a foundation to discover the pathogenesis and effective drugs.Methods Using 32 P-labeling method to determine the activity of TPK and MAPK.Using Fluo 3-AM as fluorescent probe to determine intracellular calcium of patients with type 2 DM.Using photometry to determine the concentration of NO.Results The activities of TPK,MAPK remarkably descended and the concentration of NO also greatly descended compared with healthy people.The concentration of intracellular calcium increased compared with healthy people.Conclusion The defects of the related signal molecule in insulin signal transduction of type 2 DM existed.
出处
《中国糖尿病杂志》
CAS
CSCD
2000年第5期266-268,共3页
Chinese Journal of Diabetes
