摘要
目的:观察红景天预处理对肝叶切除患者肝脏缺血-再灌注损伤的保护作用。方法;64例拟行肝叶切除的患者随机均分为对照组(A组)和红景天组(B组),B组大珠红景天胶囊连续口服5 d,最后一次于术前30 min以大珠红景天注射液加生理盐水100 ml静脉滴入,A组静脉滴入等量的生理盐水;在肝门阻断再灌注1h、再灌注6 h及再灌注24 h时抽取静脉血测定谷丙转氨酶(ALT)、谷草转氨酶(AST)及丙二醛(MDA)水平,并于再灌注1 h取肝组织进行病理检测。结果:A组和B组ALT再灌注1 h,再灌注6 h及再灌注24 h 3个时相点均升高,再灌注6 h达到峰值,但B组升高幅度较A组低,差异有统计学意义(P<0.05);AST在3个时相点也升高,于再灌注6 h达到高峰并与ALT一致;MDA 3个时相点B组升高幅度显著低于A组,差异有统计学意义(P<0.05)。再灌注1 h肝组织损害B组较A组减轻。结论:红景天预处理能抑制氧自由基的产生,对肝脏缺血-再灌注损伤具有保护作用。
Objective： To investigate the protective effects of salidroside pretreatment on hepatocyte functions after ischemia-reperfusion injury in partial hepatectomy patients. Methods： Sixty-four liver tumour patients were randomly divided into a saline control group （A）, and a salidroside pretreatment group （B）. The patients in group B were pretreated with salidroside orally for five days, and the last administration was at 30 minutes before operation by vein injection. Patients in group A were pretreated with normal saline at the same dose and time. During the operation of partial hepatectomy, hepatic blood flow was totally clamped for 10 -30 min and then released. In lh, 6h, and 24h after reperfusion ,levels of alanine aminotransferase （ATL）, aspartaste aminotransferase （AST）, and malondial dehyde （MDA） were detected. Liver tissue at 1 h after reperfusion was taken and examined on common paraffin sections with HE staining. Results： The serum levels of ALT, AST, and MDA were markedly lower in group B than those in group A （ P 〈 0. 05 ） at time points of 1 h, 6h, and 24h after liver reperfusion. Liver tissue injury and hepatocyte damage in group B were milder than those in group A at 1 h after reperfusion. Conclusions： Pretreatment with salidroside could inhibit production of oxygen free radicals, which has some protective effects to liver tissue against hepatocyte injuries caused by ischemia-reperfusion.
出处
《贵阳医学院学报》
CAS
2013年第1期41-43,49,共4页
Journal of Guiyang Medical College
基金
成都军区昆明总医院科研基金资助
编号(2007Y041C
2008Y019B)
关键词
红景天
肝
再灌注损伤
氧化应激反应
自由基
salidroside
liver
reperfusion injury
oxidative stress reaction
free radicals
