摘要
为了解我国野生动物源细小病毒VP2、NS1基因序列和进化特点,用PCR方法获得貉源细小病毒CR86106和猴源细小病毒BJ-22的目的基因片段,对其核苷酸和氨基酸序列进行测定分析。结果显示CR86106和BJ-22细小病毒基因组长均为4 269nt,其中NS1基因全长2 007nt,共编码668个氨基酸;VP2基因全长1 755nt,共编码584个氨基酸。CR86106VP2蛋白除第300位氨基酸为脯氨酸(P)以外,其余关键氨基酸位点均与猫泛白细胞减少症病毒(feline panleukopenia virus,FPLV)一致;BJ-22VP2蛋白除第323位为天冬酰胺(N)、第564位为丝氨酸(S)以外,其余关键氨基酸位点均与FPLV一致。CR86106NS1蛋白氨基酸序列与细小病毒参考毒株的相似性是98.4%～99.3%,VP2是97.6%～99.7%;BJ-22NS1蛋白氨基酸序列与细小病毒参考毒株的相似性是98.5%～99.4%,VP2是98.1%～99.3%。种系发生分析结果显示,CR86106VP2和NS1与FPLV亲缘关系较近;BJ-22NS1归到CPV分支,VP2归到FPLV分支且单独分在一支。结果表明,CR86106具有FPLV样细小病毒序列特征,BJ-22具有FPLV和CPV重组病毒特征,为猴源细小病毒的重组现象,研究结果同时也证实了基因重组在FPLV进化过程中起重要作用的推论。
In order to sequence VP2 and NS1 genes of parvoviruses isolated from wild animals in- cluding CR86106 from a raccoon dog and BJ-22 from a monkey and analyse the evolution of the vi- ruses,the VP2 and NS1 genes were amplified by PCR,their nucleotide and amino acid sequences were analysed and molecular variation. CR86106 and BJ-22 had the same nucleotide length （4 269 nt）. The NS1 gene was 2 007 nt and encoded 668 amino acids,the VP2 gene was 1 755 nt and en- coded 584 amino acids. The 300 th amino acid remanant base was P on the VP2 protein of CR86106, and the other key amino acid sites were the same with FPLV. The 323 th amino acid re- manant base was N on the VP2 protein of BJ-22,the 564th is S,and the other key amino acid sites were also the same with FPLV. The amino acid homologies of NS1 protein and VP2 protein be- tween CR86106,BJ-22 and published sequences of CPV and FPLV were as followeed： 98.4%- 99.3% for NS1 protein of CR86106,97.6%-99.7% for VP2 protein of CR86106;98. 5%-99. 4%for NS1 protein of BJ-22,98.1%-99.3% for VP2 protein of BJ-22. Phylogenetic anlysis showed that the VP2 and NS1 genes of CR86106 were closely related to FPLV. The NS1 gene Of BJ-22 was found to be phylogenetically closely related to CPV, and the VP2 gene of BJ-22 formed a monophyletic branch in FPLV cluster but as an individual branch. The result showed that CR86106 had the characteristic of FPLV-like parvovirus,and BJ-22 appeared the characteristic of re- combination virus which was the first demonstration on parvoviruse from a monkey. This research con- firmed the deduction that gene recombination played an important role in evolution of FPLV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第3期345-351,共7页
Chinese Journal of Veterinary Science
基金
公益性行业(农业)科研专项资助项目(201303042)
国家林业局野生动物疫源疫病资助项目(20120601)
