摘要
为了揭示云南元阳哈尼梯田持续大面积种植的水稻地方品种月亮谷的遗传变异,利用20个表型性状和48个SSR标记,对采自元阳哈尼梯田11个村寨的98份月亮谷和8份对照品种进行分析。结果显示,98份月亮谷的质量性状变异系数较小;数量性状变异系数较大,变异系数范围为2.55%～24.90%;48个标记共检测出114个等位变异,平均为2.38个,变幅为1～4个;有效等位变异为87.65个,平均为1.83个,变幅为1～3.02;Nei遗传多样性(I)指数平均为0.64。表型聚类将月亮谷与对照品种分成两大类,所有月亮谷为一类,8个对照品种为另一类;SSR聚类则将所有月亮谷与4份大于村寨内(37.82%)。11个村寨月亮谷可以分成5个居群,这5个类群与其村寨间地理位置的距离相关,相邻村寨之间月亮谷遗传距离小,遗传相似度高。月亮谷这种特殊的遗传变异是由于独特的哈尼梯田及其传统文化长期作用的结果,月亮谷可作为优异基因资源进一步发掘和利用。
In order to reveal the genetic variation of rice landrace Yuelianggu which has been widely grown in Yunnan Hani ’ s terraced fields for long-term,98Yuelianggu samples collected from 11villages located in Yunnan and 8rice varieties as control were analyzed by using 20phenotypic traits and 48SSR markers.The results showed that the variation coefficients for the quantitative characters were larger than those of the qualitative characters with a range from 2.55%to 24.90%.A total of 114alleles in all of Yuelianggu were found with the mean of 2.38and range from 1to 4,as well as the number of 87.65effective alleles with the mean of 1.83and range from 1to 3.02.The mean of Nei ’ s genetic diversity index（I） was 0.64.Clustering analysis based on phenotypes could divide the used materials including Yuelianggu and control varieties into two categories,with Yuelianggu belonging to one group while the other eight varieties to another one.However,clustering analysis based on SSR markers could divide the tested moterials into two categories,with one group including all Yuelianggu samples and the indica control while another one only including the japonica control.Analysis of molecular variance（AMVOA） revealed that the inter-village genetic variations of Yuelianggu consisted of 67.18%and the intra-village genetic variation contisted of 37.82%.Yuelianggu samples collected from 11villages could be divided into 5populations,which were closely related to geographical distance between the villages.Genetic distances of Yuelianggu collected from adjacent villages were small while their genetic similarities were high correspondly.The special genetic variation of Yuelianggu resulted from the unique Hani ’ s terraced fields and its traditional culture for long-term.Yuelianggu can be used as a benefit gene resource to further exploit and utilize.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2013年第2期137-144,共8页
Chinese Journal of Rice Science
基金
国家863计划资助项目(2010AA101805)
云南省科技创新强省计划资助项目(2007C0219Z)
云南省社会发展科技计划项目(2010CC009
2012CH009)
云南省人才培养资助项目(2008PY049)
关键词
哈尼梯田
水稻
遗传变异
月亮谷
Hani ’s terraced field
rice
genetic variation
Yuelianggu
