摘要
目的:通过检测miR-182在正常妊娠各期、老化胎盘组织中的表达,探讨miR-182在胎盘发育过程中的调控作用,并同时探讨miR-182与血管内皮生长因子(VEGF)蛋白表达的相关性,为寻找胎盘老化特别是胎盘提前老化发生机制提供依据。方法:采用实时荧光定量PCR方法检测42例标本(早孕绒毛和胎盘组织)中miR-182的表达情况。结果:miR-182在正常妊娠早孕绒毛组织、正常妊娠胎盘组织及老化胎盘组织中均表达。miR-182表达水平各个组间比较:早期与中期妊娠组、中期与晚期未足月妊娠组、晚期未足月与晚期足月妊娠组有显著差异(P<0.05);胎盘老化组与正常妊娠早期、中期、晚期未足月、晚期足月组有极显著差异(P<0.01);妊娠的早期、中期、晚期,miR-182的表达逐渐增多,老化胎盘组织中miR-182高水平表达。结论:miR-182与胎盘老化的发生高度相关,可能在胎盘老化机制中起重要的调控作用。
Objective： To investigate the effect of miR-182 with the development of placenta through the detection of miR-182 expression during every period of normal pregnancy and aged placental tissues, and also to provide the correlativity between miRo 182 and Vascular Endothelial Growth Factor （VEGF）. Methods： The method of real-time PCR was adopted to detect miR-182 expression of 42 samples （Chorionic villi and placental tissue）. Results： There was the expression in tissues of Chorionic villi and placenta during normal pregnancy, and also aged placenta. The comparison among different groups of miR-182 expression was as follows： groups of early pregnancy and mid-pregnancy, mid-pregnancy and late abortion pregnancy, late abortion pregnancy and late term pregnancy has significant difference （P 〈0.05）; The group of placental aging, normal pregnancy of early, mid, late abortion, and late has extremely significant difference （P〈0.01）. In early pregnancy, midpregnancy and late pregnancy, miR-182 expression increased, the level of miR-182 expression highly in aged pacental tissue. Conclusions： MiR-182 has relationship with emergence height of placental aging, which may figure out the function in placental aging system.
出处
《现代生物医学进展》
CAS
2013年第3期420-423,455,共5页
Progress in Modern Biomedicine
基金
黑龙江省博士后启动基金(LRB05-111)
黑龙江省高等学校青年学术骨干支持计划项目(1151G027)
黑龙江省青年科学基金(QC08C79)
