摘要
目的:探讨阿尔茨海默病模型大鼠海马CREB的变化以及电针对其的影响。方法:将40只SD大鼠按随机数字表分为4组,即正常组、假手术组、模型组、电针组。采用双侧海马一次性注射聚集态Aβ25～35制备AD大鼠模型,电针组选取双侧"肾俞"穴、双侧"内关"穴和"大椎"穴,接G6805-Ⅱ型电针治疗仪,选用2 Hz连续波,强度为1 mA,通电20 min,每天1次,每周6次,共治疗2周。治疗结束后,采用免疫组化法检测各组大鼠海马CREB表达的变化,并进一步分析电针对其的影响。结果:经图像分析,与假手术组比较,模型组大鼠海马CA1、CA3区CREB的表达明显降低,有显著性差异(P<0.01);电针组大鼠海马CA1区CREB的表达与模型组比较有显著提高(P<0.05),而CA3区CREB的表达虽有升高的趋势,但无统计学意义(P>0.05)。结论:电针可能通过增强海马CREB的表达,改善LTP,提高AD大鼠的学习记忆能力。
Objective：To explore the changes of cAMP - response element binding protein in the hippocampus of rats with Alzheimer＇s Disease and observe the effect of electro -acupuncture therapy on the hippocampus. Methods：Male SD rats in number 40 were randomly divided into 4 groups in average, the normal group, the sham group, the model group, and the EA group. By utili-zing condensed Aβ25 - 35 in one -time injection of bilateral hippoeampus to induce AD model rats, we selected bilat-erahn Shen- shu（BL23） ,bilat- cralm Neiguan（PC6） and Dazhui （GV14）acupoints in EA group. G6805 - Ⅱ type of electroaeupuneture treatment device was connected to needles, and then we selected the frequency of 2 Hz eontinus wave （ CW）, the intensity of 1 mA. The therapy last 20 rains, qd,6 times a week,a total of 2 weeks treatment. The expression of CREB protein in hippocampus was assayed by immunohistochemistry. Results：Compared with the sham group,the expression of CREB in hippocampus CA1 ,CA3 zone of the model group was lower, and the difference was significant（P 〈 0. 01 ） ;compared with the model group, the expression of CREB in CA1 zone of the EA group was significantly inereased（P 〈 0. 05 ）. The expression of CREB in CA3 zone of the EA group was increased,but there was no statistical significanee（P 〉0. 05）. Conclusion：Its mechanism may be achieved through the following mean ： increasing the expression of CREB in the hippocampus, enhancing LTP, in order to improve the learning - memo- ry capacity of rats with AD.
出处
《辽宁中医杂志》
CAS
2013年第3期531-533,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30772837)
关键词
电针
AD
海马
CREB
electro-acupuncture
Alzheimer＇s disease
hippocampus
cAMP-response element binding protein
