摘要
为了探讨低磷胁迫下氮素形态对玉米糖代谢以及花青苷含量的影响,以Ca3(PO4)2模拟低磷胁迫环境,采用水培方式,研究3种形态氮素营养(NO3--N、NO3--N与NH4+-N混合营养和NH4+-N)对玉米植株生物量、花青苷含量及糖代谢等的影响。结果表明:当营养液中以Ca3(PO4)2形式供应磷素时,与KH2PO4形式供应磷素相比,3种氮素处理的玉米整株生物量依次降低20.6%、15.4%和12.9%;在磷胁迫状态下,玉米茎秆的花青苷含量显著升高,同时受到氮素形态的影响,3种氮形态处理下茎秆的花青苷含量高低顺序依次为:铵态氮>铵硝混合营养>硝态氮;磷胁迫还引起玉米植株中糖分累积,与KH2PO4处理相比,3种氮形态处理的玉米植株根、茎、叶的可溶性糖含量均明显升高,且在根和茎中差异达显著性水平;在低磷胁迫时,铵态氮处理的玉米根、茎、叶中可溶性糖含量分别是硝态氮处理的1.12倍、1.22倍、1.18倍,相应的还原性糖的数值依次为1.03倍、1.37倍、1.24倍。可见,铵态氮处理的玉米生长受抑制程度最小,表现为铵态氮处理提高了植物各部分糖的含量,促进了花青苷合成,以此缓解低磷胁迫引起的代谢失调。
In order to study the effect of nitrogen forms on the sugar metabolism and anthocyanin content under phosphorus stress in maize seedling, a hydroponics experiment was carried out to study effects of nitrogen forms （NO3--N, the mixture of NO3--N＋NH4＋-N, and NH4＋-N） on maize seedling biomass, sugar and anthocyanin content under the phosphorus stress treatment by using Ca3（PO4）2 as the phosphorus form supply. The results showed that the biomass of maize plant under phosphorus stress supplied with NO3--N, the mixture of NO3--N＋NHn＋-N, and NH4＋-N was reduced by 20.6%, 15.4% and 12.9% compared with KH2PO4 treatment, respectively. The anthocyanin contents of the maize stem under low phosphorus stress were increased obviously, and simultaneously were affected forms nitrogen fertilizer. The maize stem anthocyanin contents under three kinds of nitrogen forms from high to low were NH4＋-N, the mixture of NO3--N＋NH4＋-N, and NO3--N. The sugar contents of maize plant accumulated resulted from low phosphorus stress, which the soluble sugar contents of maize root, stem and leaf supplied with three forms nitrogen fertilizer significantly increased compared with those by using KH2PO4 as phosphorus resource, moreover, the differences were remarkable in both roots and stems. Under the low phosphorus stress, the soluble sugar contents of maize root, stem and leaf using the form of NH4＋-N were 1.12 times, 1.22 times and 1.18 times higher than using the form of NO3--N, correspondingly, the times of the reducing sugar contents were 1.03, 1.37 and 1.24. Therefore, we conclude that the NH4＋-N treatment increase the sugar content of plant, promote the anthocyanin synthesis to relief the metabolic disorders, which caused by phosphorus stress, so the NH4＋-N treatment is found more tolerant to phosphorus stress as compared to the other two nitrogen forms supplied plants.
出处
《热带作物学报》
CSCD
北大核心
2013年第2期254-258,共5页
Chinese Journal of Tropical Crops
基金
安徽省长三角联合科技攻关项目(No.1101c0603046)
国家公益性行业(农业)专项经费项目(No.201103004)
安徽省高校重大科研项目(No.KJ2012ZD04)
关键词
低磷胁迫
玉米
氮素形态
糖含量
花青苷
Low phosphorus stress
Maize
Nitrogen forms
Sugar content
Anthocyani
