摘要
以14份海南黎族聚居区的山栏稻为研究材料、以原产于中国的69份亚洲栽培稻和110份普通野生稻为对照组,分别对核中SSⅡ基因、ITS基因和Ehd1基因、叶绿体中ndhC-trnV基因以及线粒体中cox3基因等5段序列进行测序,分析基因序列多样性和单倍型,并揭示海南黎族聚居区山栏稻的起源地和驯化过程。结果表明,黎族聚居区山栏稻的基因多样性低于亚洲栽培稻,而亚洲栽培稻的基因多样性低于普通野生稻;85%左右的山栏稻为偏粳型;山栏稻与广东和湖南的普通野生稻亲缘关系较近,而与海南的普通野生稻的亲缘关系较远,推测黎族的山栏稻可能起源于广东和湖南的普通野生稻。
In this study,14 accessions of upland rice（ Oryza sativa L. ） named Shanlan rice by Li ethnic peo- ple,69 accessions of Asian cultivated rice（ Oryza sativa L. ）, and 110 accessions of common wild rice（ Oryza ruff- pogon） were selected for sequencing analysis to study the origin and evolution of upland rice in Li ethnic communi- ties in Hainan province of China. Five high mutation regions of SSL7 ,ITS, and Ehdl gene from nucleus genome,the ndhC-trnV inter spacer from chloroplast genome, and cox3 gene from mitochondrial genome in rice were chosen to be sequenced. The results showed that the gene diversity of Shanlan rice was the lowest while the common wild rice was the highest and that of Asian cultivated rice was in the middle. 85 percent of Shanlan upland rice was japonica. In addition,Shanlan rice had a close genetic relationship with common wild rice from Guangdong and Hunan province but not Hainan province,which inferred that Shanlan rice might be originated from common wild rice from Guang- dong and Hunan province.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第2期202-207,共6页
Journal of Plant Genetic Resources
基金
农业野生植物资源保护利用技术研究与示范(201003021)
转基因水稻环境安全评价技术(20082x08011-001)
高等学校学科创新引智计划(B08044)
中央民族大学"985"工程项目(MUC98504-14
MUC98507-08)
关键词
黎族
山栏稻
SNP
基因多样性
起源
演化
Li ethnic
Shanlan rice
SNP
gene diversity
origin
evolution
