摘要
研究通过筛选亲本间具有多态性的SSR引物,对材料的亲本和后代进行分子鉴定,获得材料间的系谱关系。供试的23份材料通过分子鉴定得到:4份后代材料可能为伪杂种后代;1对基因型相同的姊妹系;另外确定12份后代材料可能的一个亲本材料和1份后代材料的两个可能亲本。研究结果表明,在亲本基因型纯合的情况下,采用分子标记有助于大豆育种的系谱分析与鉴定。
In order to solve practical problems in soybean breeding, a set of SSR primer were used to screen the polymorphism. Then 23 tested materials were genotyped to reveal their relationship. The results showed that four lines were pseudo-hybrid; one pair of lines were potential sister lines with exactly the same genotypic profiles; 12 lines were carrying one parental genetic background and one had both parental backgrounds. Therefore, molecular markers should be useful in detecting the soybean crossing or pedigree relationship.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第10期91-95,共5页
Journal of Northeast Agricultural University
基金
国家转基因专项(2009ZX08009-013B)
现代农业产业技术体系大豆产业体系(200903003)
关键词
大豆
育种
系谱
分子鉴定
soybean
breeding
pedigree
molecular identification
