摘要
目的观察蛛网膜下腔注射胶质细胞源性神经营养因子（GDNF）对脊神经结扎（SNL）大鼠脊髓背角Fos蛋白的影响。方法将L5-6。脊神经结扎sD雄性大鼠随机分为对照组、假手术组、单纯SNL组（蛛网膜下腔注射生理盐水）、GDNF治疗组（蛛网膜下腔注射GDNF）。每组又根据处死大鼠的时间不同各分为3个亚组：术后3、7、14d。于大鼠脊神经结扎后不同时间点取脊神经结扎侧脊髓，测定脊髓背角处Fos蛋白的表达。结果与对照组和假手术组比较，SNL组脊髓背角Fos蛋白表达在术后3d（分别为1．00±0．00、1．08±0．12、4．62±0．52）开始增高，一直持续至术后14d（分别为1．00±0．00、1．15±0．21、4．43±0．84）（P〈0．01或P〈0．05）；GDNF组Fos蛋白表达（1．82±0．51）明显低于SNL组，持续至术后14d（1．61±0．26）（P〈0．01或P〈0．05）。结论大鼠脊髓背角中F0s蛋白参与了SNL所致神经病理性疼痛的发生机制，蛛网膜下腔注射GDNF大鼠减轻神经病理性疼痛机制与其抑制脊髓背角Fos蛋白表达有关。
Objective -To study the effect of glial cell line-derived neurotrophic factor （GDNF） on Fos protein expression in the spinal dorsal horn of neuropathic pain rat model. Methods Adult Spra- gue-Dawley male rats were randomly divided into normal control group, sham operation group, spinal nerve ligation （SNL） group and SNL combined with treatment of GDNF group. Every group was divided into three sub-groups： SNL 3, 7 and 14 days （n = 10 in each group）. Fos protein expression in the spinal dor- sal horn was examined by using inununohistochemistry and Western blotting. Results Compared with nor- mal control and sham operation groups, the Fos protein expression in the spinal dorsal horn of the rats in SNL group was increased significantly （ 1.00±0. 00, 1.08±0. 12, and 4. 62±0.52 respectively）. As compared with the rats in SNL group, there was an apparent decrease in GDNF treatment group three days later （ 1.82 ±0. 51 ）. Conclusion Fos protein in the spinal dorsal horn of rats was involved in the mecha- nism of neuropathic pain. Intrathecal administration of GDNF could inhibit Fos protein expression in the soinal dorsal horn.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第3期612-614,共3页
Chinese Journal of Experimental Surgery
