摘要
以无籽西瓜"蜜童"无菌苗子叶为外植体,研究了种子灭菌方法、子叶的取材部位、黑暗培养等不同条件对其不定芽诱导的影响。结果表明:相同培养条件下,"蜜童"无菌苗子叶不定芽的分化能力由近轴端至远轴端逐渐降低,外植体的离体黑暗培养不能促进不定芽的诱导,相反具有一定抑制作用。
Using seedling-derived cotyledon of triploid watermelon , Mitong＇ as exp!ants, the influences of sterilization method of seed sterilization, different sampling areas and dark culture on adventitious shoots induction were studied. The results showed that under the same cultivation condition, the： differentiation of adventitious shoots from proximal cotyledon was more easily than from other areas of cotyledon, and adventitious shootS＇ differenlciation ~bility：teducgd gradually from the nearly hypocotyl area to the distal end. The study indicated that dark culturing could not improve （sometimes reduces） the cotyledon adventitious shoot induction of triploid Watermelon.
出处
《北方园艺》
CAS
北大核心
2013年第5期103-105,共3页
Northern Horticulture
基金
武汉市科技攻关计划资助项目(201021112428-9)
武汉市农业科学技术研究院创新资助项目
关键词
无籽西瓜
灭菌方法
子叶
取材部位
暗培养
triploid watermelon
sterilization method
cotyledon
sampling area
dark culture
