摘要
目的通过白细胞介素1-α（IL-1α）的刺激,观察ELAM-1对体外培养的猪眼小梁细胞MMP9表达的影响,以期发现青光眼发病的病理生理机制.方法取猪眼小梁组织进行小梁细胞体外培养,取传3－5代的猪眼小梁细胞,分人重组IL-1α组、ELAM-1组、IgG组和对照组, ELAM-1抗体及IgG进行干预,用免疫荧光法追踪抗体,采用RT-PCT技术检测小梁细胞MMP9-mRNA的变化.结果体外培养的猪眼小梁细胞大约2周开始从组织块表面爬出. RT-PCR结果显示ELAM-1组、IgG组的MMP9-mRNA相对表达量明显低于IL-1α组;IgG组MMP9-mRNA的相对表达量低于ELAM-1组;IgG组MMP9-mRNA相对表达量低于对照组.结论 ELAM-1抗体能使体外培养的猪眼小梁细胞MMP9-mRNA的表达量明显减少, ELAM-1调控猪眼小梁细胞MMP9的表达,进而影响细胞外基质的代谢和眼压的变化.
Objective To observe the influences of ELAM-1 on MMP9 of cultured porcine trabecular cells stimulated by IL-1αin vitro,in order to detect the pathogenesis of glaucoma .Methods This was an experimental re-search.Trabecular cells were cultured in vitro,porcine trabecular cells of 3~5 generation were selected and divided into control group and experimental group .Antibodys of ELAM-1 and IgG were used as intervene factor ,traced them by im-munofluorescence method and detected expression of MMP 9 of cultured porcine trabecular cells by RT-PCR.Results In vitro cultured porcine trabecular cells started climbing out from the tissue surface about 2 weeks later.The relative ex-pression of MMP9-mRNA in ELAM-1 group and IgG group was lower than IL-1 group;IgG group was lower than ELAM-1 group and control group.Conclusion The antibody of ELAM-1 can reduce expression of MMP9 of in vitro cultured porcine trabecular cells.So ELAM-1 adjust the expression of MMP9 and effect metabolize of ECM and IOP.
出处
《潍坊医学院学报》
2012年第6期458-460,I0001,共4页
Acta Academiae Medicinae Weifang
