摘要
目的:探讨构树叶总黄酮(TFBP)对人肝癌细胞(HepG-2)增殖和凋亡的作用。方法:采用细胞增殖实验(MTT法),取对数生长期HepG-2,随机分为药物组和对照组,用3,6,9,12 g.L-1不同质量浓度构树叶总黄酮作用HepG-2,分别干预24,48,72 h。通过流式细胞仪检测在以上质量浓度下作用细胞48 h后的细胞凋亡率,Western blont法检测以上质量浓度构树叶总黄酮作用细胞48 h后Bcl-2基因、促凋亡基因Bax表达的情况。结果:各质量浓度构树叶总黄酮对HepG-2的生长有显著的抑制作用,呈明显的时间和剂量依赖性,经不同质量浓度的构树叶总黄酮作用48 h后HepG-2凋亡率显著增加,药物组与对照组比较为(0.478±0.085 vs 0.498±0.014;0.354±0.004 vs 0.498±0.014;0.218±0.075 vs 0.498±0.014)有显著性差异,P<0.05,抑癌基因Bcl-2表达量呈逐渐下降的趋势,促凋亡基因Bax表达量随着质量浓度的升高表达量呈现上升趋势。结论:构树叶总黄酮可以有效的抑制HepG-2的生长,诱导细胞凋亡,其诱导该细胞凋亡的作用可能是通过下调抑癌基因Bcl-2的表达,可为肝癌的生物学治疗提供新的方法。
Objective： To investigate the effects of total flavonoids of Broussonetia papyrifera on growth and apoptosis of hepatocellular carcinoma HepG-2 cells. Method： The MTT assay was used, and the hepatocellular carcinoma HepG-2 cells in logarithmic phase of growth were randomLy divided into drug group and control group, with the concentration of 3, 6, 9, 12 g·L-1 total flavonoids of B. papyrifera in hepatocellular carcinoma HepG-2 cells, intervention durations were 24, 48, 72 h accordingly.The percentage of apoptotic cell were detected by FCM.The expression of protein Bcl-2 was analyzed by Western blot. Result： Inhibition rate of the hepatocellular carcinoma HepG-2 was obviously enhanced along with increase of the total flavonoids of B. papyrifera concentration, the effect is also showed in time course.Flow cytometry study revealed, with the concentration increased, the percentage of apoptotic cells raised after 48h.The expression of protein Bcl-2 was down-regulated along with increase of the total flavonoids of B. papyrifera concentration. Conclusion： The quartering can effectively inhibit tumor cell growth and induced tumor cells into apoptosis.It may be associate with the low expression of protein Bcl-2 in hepatocellular carcinoma cell line HepG-2.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第6期270-273,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技特派员专项(桂科攻09321056
桂科能129825-21)
广西教育厅项目(201202ZD065)
桂林市科技攻关项目(20110106-5
20120105-5
20120105-16
20120105-8)
关键词
构树叶总黄酮
肝癌
增殖
凋亡
total flavonoids of Broussonetia papyrifera
hepatocellular carcinoma
proliferation
apoptosis
