摘要
目的观察富氢液(HRS)对脓毒血症大鼠肠损伤的影响。方法 24只雄性SD大鼠随机均分为三组:假手术组(Sham组)、盲肠结扎穿孔(CLP)组及CLP+HRS组。CLP+HRS组于CLP后0、6、18h腹腔分别注射HRS5ml/kg,Sham组及CLP组于上述时点腹腔注射生理盐水5ml/kg。CLP或假手术24h后检测肠组织TNF-α、IL-6、高迁移率族蛋白1(HMGB1)、丙二醛(MDA)水平,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性及肠组织损伤程度。结果与Sham组比较,CLP组和CLP+HRS组大鼠肠组织TNF-α、IL-6、HMGB1及MDA水平明显升高(P<0.05),SOD和GSH-PX活性明显降低(P<0.05);与CLP组比较,CLP+HRS组肠组织IL-6、HMGB1和MDA水平明显降低,SOD和GSH-PX活性明显升高(P<0.05),肠组织损伤程度减轻。结论 HRS通过抑制肠组织炎症反应及氧化应激,减轻肠组织损伤程度。
Objective To observe the effects of hydrogen-rich saline （HRS） on intestinal injury in a rat model of sepsis. Methods A in vivo rat model of sepsis was established by cecal ligation and puncture （CLP） in our present study. Twenty-four male SD rats were randomly assigned to group Sham, group CLP and group CLP＋ HRS. At 0, 6 and 18 h after operation, rats received an intraperitoneal injection of HRS of 5 mi/kg （group CLP＋HRS）, or 5 ml/kg of normal saline （groups sham and CLP）. Intestinal levels of TNF-α, IL-6, high mobility group box-1 protein（HMGB1） and malondialdehyde （MDA）, and activities of superoxide dismutase （SOD）, glutathione peroxidase GSH-PX （GSH-PX） and catalase （CAT） were assessed at 24 h after operation. Results Compared with group Sham, TNF-α, IL-6, HMGB1 and MDA were increased while SOD and GSH-PX were decreased in groups CI.P and CLP ＋HRS. Compared with group CLP, group CLP ＋ HRS had significantly improved intestinal pathological injuries, decreased intestinal levels of IL-6, HMGB1 and MDA, and increased activities of SOD and GSH-PX （P 〈 0. 05 ）. Conclusion HRS ameliorates intestinal injury via inhibiting inflammatory responses and oxidative stresses in a rat model of sepsis.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2013年第2期178-180,共3页
Journal of Clinical Anesthesiology
基金
江苏省第五批"六大人才高峰"课题(No:2008093)
江苏大学临床科技发展基金项目(No:JLY2010052)
江苏省常州市卫生局科技项目(No:WZ201044)
关键词
脓毒症
氢气
肠
炎性介质
氧化应激
Sepsis
Hydrogen
Intestine
Inflammatory mediator
Oxidative stress
