摘要
观察正常妊娠妇女和子痫前期疾病患者外周血CD4+T细胞中Th17细胞和特异性转录因子维A酸相关孤独受体(retinoid-related orphan nuclear receptor,RORγt)的表达和意义。实验组为子痫前期疾病患者25例,正常妊娠妇女20例,未孕妇女20例。采集研究对象外周血,酶联免疫吸附试验(ELISA)检测Th17细胞相关细胞因子IL-17A,IL-6,TNF-α的表达,Ficoll法分离外周血单个核细胞(PBMC),免疫磁珠分选CD4+T淋巴细胞,逆转录-聚合酶联反应(RT-RCR)半定量检测CD4+T细胞中Th17细胞特异性转录因子RORγt的表达,流式细胞术检测CD4+IL-17+T细胞比例。子痫前期患者外周血清中IL-6、IL-17A的含量分别为(31.72±13.34)ng/L、(2.61±1.64)ng/L,高于正常妊娠组水平,差异有显著统计学意义(P<0.01),TNF-α在子二组间的表达分别为(18.00±8.64)ng/L和(11.69±3.68)ng/L,差异有统计学意义(P<0.05);RORγt mRNA在子痫前期组的表达高于正常妊娠组,净光密度值差异有显著统计学差异(P<0.01),CD4+IL-17+T细胞在子痫前期组的表达为(1.83±0.42)%,高于正常妊娠组(0.87±0.26)%,差异有显著统计学意义(P<0.01)。子痫前期患者外周血CD4+T细胞中RORγt mRNA、Th17细胞以及Th17细胞相关细胞因子表达异常,可能在疾病的发病机理中起到重要作用。
To observe the expression of CD4＋ IL-17＋ T （Th17） cells and RORγt mRNA in preeclampsia patients and normal preganancy women, 25 preeclampsia patients, 20 normal pregnancy subjects and 20 non-pregnant healthy controls were included. IL-6, IL-17A and TNF-α amount in serum were measured by ELISA. CD4＋T lymphocytes were separated using the magnetic BD IMag Cell Separation System according to the manufacturer＇s instructions. The expression of RORγt gene mRNA were detected by RT-PCR technique. The proportion of CD4＋ IL-17＋ T was determined by flow eytometric technique. The levels of IL-17A and IL-6 and TNF-α in preeclampsia sera were significantly higher than those in normal pregnancy, and the level of RORγt mRNA were significantly higher than that in normal pregnancy （P〈0.01）. The proportion of CD4＋ IL-17＋ T cells in preeclampsia patients was （1.83 ± 0.42）%, stastistieally higher than that in the normal, pregnancy. （P〈0.01）. Thus RORγt mRNA, Thl7 cells and Th17-related cytokines may play important roles in the pathogenesis of preeclampsia.
出处
《现代免疫学》
CAS
CSCD
北大核心
2013年第2期127-130,共4页
Current Immunology
基金
江苏省南通市社会发展基金项目(S2010053)
江苏省妇幼保健重点资助项目
