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淫羊藿苷对内毒素抑制牙周膜细胞ALP表达的影响 被引量:6

Effects icariin of on the alkline phosphatase activity of human periodontal ligament cells inhibited by LPS
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摘要 目的:探讨淫羊藿苷对人牙周膜细胞(periodontal ligament cells,PDLC)增殖及对内毒素(Lipopolysaccha-ride,LPS)干扰下碱性磷酸酶(alkaline phosphatase,ALP)的影响。方法:原代培养PDLC,MTT法检测不同浓度(0、10-5~10-9mol/L)淫羊藿苷对PDLC增殖的影响;RT-PCR、对硝基苯酚法测定淫羊藿苷对LPS抑制PDLC的ALP mRNA表达和分泌的影响。结果:淫羊藿苷在一定浓度下(10-6~10-7mol/L)可促进PDLC增殖;10μg/mL LPS可抑制PDLC的ALP活性;加入10-6mol/L淫羊藿苷干扰后,对LPS抑制PDLC的ALP有拮抗作用,可提高其mRNA表达和活性。结论:淫羊藿苷在一定浓度时可促进PDLC增殖;可能通过拮抗LPS抑制其ALP的活性。 Objective: To investigate the effects of icariin (ICA) on proliferation of huaman periodontal ligament cells (human periodontal ligament cells, hPDLC) and the alkaline phosphatase (alkaline phosphatase,ALP) activity inhibited by LPS. Method: HPDLC were cultured in virto and stimulated with icariin with different concentrations (0,10^-5,10^-6,10^-7,10^-8, 10^-9 mol / L)) for 96 hours.The ability of proliferation of HPDLC was detected by MTT method.The activity of alkaline phos- phatase and the expression of alkaline phosphatase mRNA was seperately determined by p-Nitropbnyl phosphate (pNPP) method and RT-PCR after being treated with icariin at the concentration of 10^-6 mol / L inhibited by LPS. Result: icariin had a dose-dependent effect on the proliferation the hPDLC in a suitable concentration range from 10^-6~10^-8 mol / L. The alka- line phosphatase activity was remarkably inhibited by incubation of PDLC with 10 υg / mL LPS and was improved in the presence of icariin at the concentration of 10^-6 mol / L. Conclusion: Ieariin have the effect on the proliferation of PDLC and improve the ALP activity inhibited by LPS, which could held for Peri-at)ical tissue regeneration.
出处 《临床口腔医学杂志》 2013年第3期137-140,共4页 Journal of Clinical Stomatology
基金 黑龙江省自然基金项目(41400649-6-12281)
关键词 淫羊藿苷 牙周膜细胞 增殖 碱性磷酸酶 内毒素 Icariin PDLC proliferation alkaline phosphatase lipopolysaceharide
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  • 1王晓敏,于世凤,庞淑珍,刘忠厚.阿仑膦酸盐诱导破骨细胞凋亡FAS基因的表达[J].中国骨质疏松杂志,1998,4(4):30-33. 被引量:5
  • 2王晓敏,于世凤,刘忠厚.阿仑膦酸钠抑制破骨细胞体外吸收的研究[J].中国骨质疏松杂志,1999,5(2):63-66. 被引量:4
  • 3陈坤,于世风,史凤芹,庞淑珍.黔岭藿对体外培养的破骨细胞作用的研究[J].中国骨质疏松杂志,1996,2(3):59-61. 被引量:66
  • 4张润荃,史凤芹,庞淑珍,于世风.补骨脂对分离破骨细胞作用研究[J].现代口腔医学杂志,1995,9(3):136-138. 被引量:52
  • 5史凤芹,于世凤,张润荃,庞淑珍.大黄对破骨细胞性骨吸收作用的研究[J].现代口腔医学杂志,1995,9(4):193-195. 被引量:13
  • 6[1]Yano HK, Takamatsu N, Umeda M,et al.Prevalence of Bacteroides forsythus, Porphyromonsa gingivalis and Actinobacillus actinomycetemcomitans in subgingival microflora of Japanese patients with adult and rapidly progressive periodontitis [J].J Clin Periodontol, 2000, 27:597
  • 7[2]Ximenez LA, Haffajee AD, Socransky SS. Comparison of the microbiota of upra- and subgingival plaque in health and periodontitis [J].J Clin Periodontol, 2000,27: 648
  • 8[3]Yasuko Y, Takao K, Kenichi S, et al.Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with Bacterial lipopolysaccharides [J].Infect Immu,1995,63(9): 3576
  • 9[4]Kawase TS, Sato S,Mioke K.Alkaline phosphatase of human periodontal ligament fibroblast-like cells[J].Adv Dent Res, 1998,2:234
  • 10Tsuda E, Goto M, Mochizuki S,et al. Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis[ J], Biochem Biophys Res Commun, 1997,234( 1 ) :137 - 142.

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