摘要
目的应用细菌纤维素膜修补兔硬脑膜缺损,观察植入后早期局部组织学改变以及炎症因子表达情况。方法 24只新西兰兔随机分为A、B两组,每组12只。所有动物经切除双侧硬脑膜制备2 cm×1 cm的缺损。A组动物的右侧硬脑膜缺损采用细菌纤维素膜修补,左侧硬脑膜缺损则不予修补,于术后第30、90、180天采集标本,行组织学检查。B组动物的右侧硬脑膜缺损采用细菌纤维素膜修补,左侧硬脑膜缺损以人工硬膜修补,于术后第7、14、21天采集修补部位组织,采用RT-PCR技术检测促炎症细胞因子白介素1β(IL-1β)、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)mRNA的表达。结果所有实验动物均存活,切口无感染。在A组的细菌纤维素膜修补侧,细菌纤维素膜均匀覆盖脑表面,与脑组织无粘连;外侧面纤维结缔组织增生,内侧面成纤维细胞排列均匀,新生血管形成,炎症反应轻微;在A组的未修补侧,大脑与皮下组织直接粘连。RT-PCR检测结果显示:术后各时间点,B组细菌纤维素膜修补侧IL-1β和IL-6 mRNA表达水平均显著低于人工硬膜修补侧,两侧TNF-αmRNA表达水平相近。结论采用细菌纤维素膜修复硬脑膜缺损,不易与脑组织形成粘连且早期炎症反应轻微。细菌纤维素膜可能成为理想的硬脑膜替代材料。
Objective To patch up the dural defects of rabbits with bacteria cellulose membrane, and observe the early pathological change and inflammatory response after implantation. Methods Twenty-four New Zealand rabbits were randomly divided into group A and group B, with 12 rabbits in each group. The bilateral dura matter of rabbits were removed, and defects of 2 cm ~ 1 cm were prepared. In group A, the right dural defects were repaired with bacteria cellulose membrane, no repair was made on the left dural defects, and samples were taken 30 d, 90 d and 180 d after operation for pathological examinations. In group B, the right dural defects were repaired with bacteria cellulose membrane, the left dural defects were repaired with artificial dura matter, and the tissues on the repaired sites were collected on day 7, 14 and 21 after operation for determination of expression of interleukin-1β （ IL-1β）, interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） mRNA by RT-PCR. Results All rabbits survived without the presence of wound infection. On the patched side of group A, bacteria cellulose membrane evenly covered the surface of brain without adhesion and visible inflammatory cells, fibrous connective tissues proliferated on the outer side of bacteria cellulose membrane, fibroblasts uniformly distributed in the inner side, and new blood vessels appeared. On the unpatehed side of group A, the brain was directly connected with subcutaneous tissues. In group B, RT-PCR revealed that the expression of IL-1β and IL-6 mRNA in bacteria cellulose membrane was significantly lower than that in artificial dura matter at each time point, while the expression of TNF-α mRNA of both sides was similar. Conclusion Bacterial cellulose membrane may avoid the adhesion to the brain tissues and has slighter early inflammatory response in repair of dural defects. Bacterial cellulose membrane may be used as the ideal substitute material for dura matter.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期280-284,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海交通大学医工(理)交叉研究基金(YG2010MS38)
中国博士后基金(20080430659)~~
关键词
细菌纤维素膜
人工硬脑膜
炎症反应
替代材料
bacterial cellulose membrane
artificial dura matter
inflammation
substitute material
