摘要
采用聚合酶链式反应(PCR,Polymerase Chain Reaction)、变性梯度凝胶电泳(DGGE,Denaturing Gra-dient Gel Electrophoresis)与实时荧光定量聚合酶链式反应(Q-PCR,Quantitative PCR)相结合的综合分析技术,研究了青藏高原洱海、青海湖、尕海1、尕海2、小柴旦湖沉积物硫酸盐还原菌多样性及丰度。研究结果显示,5个盐湖沉积物中dsrB(编码亚硫酸还原酶β亚基,为硫酸盐还原菌所共有)基因的丰度为每克沉积物1.71×108～1.55×109拷贝,与盐度无明显相关性;所获得的dsrB基因序列分属于3个科:Desulfobacter-aceae,Desulfobulbaceae和Peptococcaceae。其中,Desulfobacteraceae科是主要类群。硫酸盐还原菌多样性(DGGE结果)与盐度呈现出负相关性。故在所研究的盐湖中,盐度可能不是影响硫酸盐还原菌种群分布的唯一因素,可能还受其它未知环境因素的影响,有待于进一步研究。
The abundance and diversity of sulfate-reducing bacteria in sediments collected from six lakes （Erhai Lake, Qinghai Lake, Gabail Lake, Gahai2 Lake, Xiaochaidan Lake, and East Dabsan Lake） on Qinghai-Tibet Plateau were investigated by using an integrated approach including polymerase chain reac- tion（ PCR）, denaturing gradient gel electrophoresis （DGGE）, and quantitative PCR （qPCR）. The re- suits show that the dsrB gene （ encoding for ~3-subunit of the dissimilatory sulfite reductase） abundance in the studied saline lakes ranges from 1.71 x l0s to 1.55 x 109 copies per gram of sediments, which did not show apparent relationship with salinity; The retrieved DGGE band sequences were affiliated with Desul- fobacteraceae, Desulfobulbaceae and Peptoeoccaceae, and Desulfobacteraceae sequences were dominant. The DGGE-based dsrB gene diversity decreased as salinity increase. Taken together, salinity may not be the sole factor controlling the abundance and diversity of sulfate reducing bacteria in Qinghai-Tibetan lakes, but other unknown environmental factors may contribute, which awaits further investigation.
出处
《盐湖研究》
CSCD
2013年第1期7-13,共7页
Journal of Salt Lake Research
基金
国家自然科学基金青年科学基金项目(41002123)
国家自然科学基金重点项目(41030211)
国家自然科学基金创新群体项目(41121001)
