摘要
目的建立家兔血浆中新藤黄酸的HPLC测定法,并研究其药代动力学。方法家兔静脉注射新藤黄酸后于不同时间点取血,经HCl酸化,乙酸乙酯提取处理,采用Phenomenex Luna C18色谱柱,以乙腈-0.1 mol.L-1醋酸铵-醋酸(80∶20∶0.1)为流动相;柱温:30℃,流速:1.0 mL.min-1,检测波长:355 nm;测定血浆中的新藤黄酸的浓度,并用非房室模型估算药代动力学参数。结果新藤黄酸在0.518～266 mg.L-1线性关系良好(r=0.9995),回收率大于90%,日内、日间精密度(RSD)均小于15%,其消除半衰期(t1/2)为23.38 min,分布容积(V)为0.13 L.kg-1,药-时曲线下面积(AUC0-∞)为1107.00 mg.min.L-1。结论该法可用于新藤黄酸的药代动力学研究,新藤黄酸在家兔体内消除迅速,滞留时间短。
Objective To establish a HPLC method for the measurement of neogambogic acid concentration in rabbit plasma, and to study the pharmacokinetics of neogambogic acid in rabbits. Methods The plasma samples were collected at diferent time after intravenous injection of single dose of neogambogic acid, and then were acidified with HC1, extracted with ethyl acetate and evaporated for dryness. The residuals were dissolved with mobile phase and injected into Phenomenex Luna C18 column. The mobile phase consisted of aeetonitrile-0.1 mol＇L-J ammonium acetate-acetic acid（80 ： 20 ： 0.1） , and was delivered at the flow rate of 1.0 mL＇min-1. The elute was detected at UV 355 nm.The column temperature was 30 ~C. The pharmaeokinetic parameters were assessed with non-compartment model. Results The standard curve was linear in the range of 0.518-266 mg·L-1 （r=0.9995）, and the recovery of neogambogic acid was over 90 %. The relative standard intra-day and inter-day deviation was less than 15% . The pharmacokinetic re- sults showed that the drug was rapidly eliminated from plasma and elimination half life （tv2） was 23.38 min, the apparent volume of distribution was 0.13 L·kg-1. The AUC0-∞ was 1107.00 mg·min＇L-1. Conclusion The established HPLC method is suitable for the pharmacokinetic study of neogambogic acid. The elimination of neogambogic acid is fast in rabbits, and the retention time is short.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第2期163-166,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省高等学校科技创新团队项目(06CXTD004)
广东省科技计划重点项目(2008A030101002)
东莞市科技计划高等院校和科研机构项目(2007108101080)
