摘要
目的评价电针预处理对局灶性脑缺血再灌注大鼠皮质神经元磷酸化信号转导及转录激活因子3（pSTAT3）蛋白表达的影响。方法成年雄性SD大鼠45只，体重280～320g，采用随机数字表法，将其随机分为假手术组、局灶性脑缺血再灌注组和电针预处理组（n=15）。局灶性脑缺血再灌注组采用线栓阻塞颈总动脉和颈外动脉24h恢复灌注的方法制备大鼠局灶性脑缺血再灌注模型。电针预处理组电针刺激百会穴30min，处理后24h时制备模型，再灌注24h时进行神经行为学评分，随后处死大鼠取脑测定脑梗死体积，计算脑梗死体积百分比，采用免疫荧光染色和WeSternblot法检测缺血半暗带皮质神经元pSTAT3蛋白的表达。结果与假手术组比较，局灶性脑缺血再灌注组和电针预处理组神经行为学评分降低，脑梗死体积百分比增加，皮质神经元pSTAT3蛋白表达上调（P〈0．05）；与局灶性脑缺血再灌注组比较，电针预处理组神经行为学评分升高，脑梗死体积百分比减少，皮质神经元pSTAT3蛋白表达上调（P〈0．05）。结论电针预处理通过上调皮质神经元pSTAT3蛋白的表达减轻大鼠局灶性脑缺血再灌注损伤。
Objective To investigate the effects of electroacupuncture （EA） pretreatment on the expression of phosphorylated signal transducer and activator of transcription 3 （pSTAT3） in cortical neurons in a rat model of local cerebral ischeinia/reperfusion （I/R） injury.Methods Forty-five adult male Sprague-Dawley rats, weighing 280-320 g, were randomly divided into 3 groups （ n = 15 each） ： sham operation group, I/R group and EA pretreatment group. Focal cerebral ischemia was induced by middle cerebral artery occlusion for 120 rain, followed by 24 h of reperfusion. EA of Baihui acupoint lasting 30 min was performed and then the model of focal cerebral I/R was established 24 h later in EA group. Neurological function was assessed and scored at 24 h of reperfusion. The rats were then sacrificed and brains removed for detection of the cerebral infarct volume and expression of pSTAT3 （Ser727） in cortical neurons in ischemic penumbra by immunofluorescenee and Western blot. Results Compared with sham operation group, the neurological function score was decreased, the infarct volume was increased, and the expression of pSTAT3 （Ser727） was up-regulated in groups I/R and EA （P 〈 0.05）. Compared with I/R group, neurological function score was increased, the infarct volume was decreased, and the expression of pSTAT3 （Ser727） was up-regulated in group EA （ P 〈 0.05）. Conclusion EA pretreatment reduces focal cerebral I/R injury through up-regulating pSTAT3 expression in cortical neurons in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第1期116-118,共3页
Chinese Journal of Anesthesiology
关键词
电针
再灌注损伤
大脑皮质
STAT3转录因子
Electroacupuneture
Reperfusion injury
Cerebral cortex
STAT3 transcription factor
