摘要
目的探讨急性ITP患儿外周血单个核细胞Th1／Th2类细胞因子的基因表达水平及临床意义。方法选择在聊城市人民医院儿科住院的30例急性ITP患儿为ITP组，同期在聊城市人民医院体检健康者20例为健康对照组，采用反转录聚合酶链反应（RT—PCR）技术，分别检测ITP组患儿治疗前后Th1／Th2类细胞因子基因表达水平，并与健康对照组比较。结果治疗前ITP患儿外周血单核细胞Th1类细胞因子（IFN-1和IL-2）基因阳性表达率分别为3．3％，、3．3％，较健康对照组明显降低（P均〈0．05）；Th2类细胞因子基因（IL-4、IL-6、IL。10）阳性表达率分别为33．3％、43．3％、40．0％，较健康对照组升高（P均〈0．05）。治疗后Th1类细胞因子基因表达水平上升，分别为16．7％、23．3％；而Th2类细胞因子基因表达水平下降，分别为10．0％、23．3％、20．0％，与健康对照组比较差异均无统计学意义。结论ITP是Th2细胞功能占优势的自身免疫性疾病，Th1向Th2漂移的细胞免疫功能异常可能在ITP的发病中起重要作用。
Objective To study the expression patterns of Thl and Th2 cytokine genes in childhood acute idiopathic tbrombocytopenic purpura（ITP） and its clinical value. Methods Peripheral blood samples from 30 patients with acute ITP before and after treatment （ ITP group ） and those from 20 normal heahhy subjects （ healthy control group） were collected （healthy control group）, and reverse transcriptases polymerase chain reaction was performed to determine the mRNA expressions of Thl and Th2 cytokine genes before and after treatment, which were compared with those of the health controls. Results The positive rate of expression levels of Thl cytokine genes in samples from ITP patients （3.3% ,3.3 % ） were significantly lower than those from healthy control group （all P 〈 0.05 ） and increased to normal level after treatment （16.7% ,23.3 % ）. In contrast,Th2 eytokine genes （IL-4, IL-6, IL-10）in the samples from the ITP patients（ 33.3% ,43.3% ,40.0% ） were significantly higher than those from the healthy control group （ all P 〈 0.05 ） and decreased after treatment （ 10.0%, 23.3%, 20.0% ）. Conclusions Such data indicate that ITP is a Th2 cell predominant autoimmune disease, and the abnormal immunity due to Thl/Th2 shift is significant in the pathogene- sis of ITP.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第3期214-216,共3页
Chinese Journal of Applied Clinical Pediatrics
关键词
特发性血小板减少性紫癜
TH1
TH2
细胞因子
基因
Idiopathic thrombocytopenic purpura
Type I and type II helper T eells
Cytokine
Gene
