摘要
以处于性腺发育期为Ⅲ期、IV期和V期的河川沙塘鳢(Odontobutis potamophila)雌鱼为研究对象,采用碱不稳定性蛋白结合磷法测定该鱼肝脏、血清和卵巢中的磷含量,以磷的含量来反应卵黄蛋白原的含量。结果显示,在河川沙塘鳢雌鱼的性成熟过程中,肝脏中的VTG含量先升后降,在IV期达到最高,其含量为(39.91±3.80)mg.g-1,在Ⅲ期和Ⅳ期,VTG合成比较活跃;血清中的VTG含量逐渐增加,从Ⅲ期的(15.45±1.77)mg.mL-1增加到V期的(33.31±5.57)mg.mL-1;卵巢中的VTG含量也逐渐增加,Ⅲ期到Ⅳ期增长速度较快(p<0.05),是VTG沉积的最重要时期,V期达到高峰,为(422.25±29.98)mg.g-1。研究揭示河川沙塘鳢性成熟过程中,VTG的合成活跃,转运高效,为其大量沉积奠定基础,保证了胚胎发育及后续发育的营养需要。
The female fishes of Odontobutis potamophila in the experiment were used as the research object, which were in the gonadal development stage Ⅲ to stage V. The levels of V TG in the serum, liver and ovary of Odontobutis potamophila were performed by an indirect method, measuring the alkali-labile phosphorus content of the lipid-free protein fraction. The levels of phosphorus can reflect the VTG levels indirectly. In the experiment, the phosphorus levels in serum, liver and ovary of Odontobutis pot- amophila were measured by the chemical response with the aqueous trichloroaeetic acid （TCA）. The results showed that the VTG level in the liver increased at first, and then decreased, the highest level was at stage Ⅳ, which was （39.91±3.80）mg·g^-1. At the stage Ⅲ and stage Ⅳ, the synthesis of VTG was very active. The level of VTG in the serum increased gradually, the VTG level from （15.45±1.77） mg·mL^-1 at the stage Ⅲ increased to （33.31±5.57） mg·mL^-1 at the stage V. The level of VTG in the ovary also increased gradually, during the stage Ⅲ to stage IV, the growth was faster, which is the most important stage of the deposition of VTG, the highest level was （422.25±29.98） mg·g^-1 at the stage V. The results indieated that the active synthesis and highly-efficient transport of VTG can lay the foundation of its vast deposits, and would assure the nutritional requirements of embryonic development and subsequent development.
出处
《重庆师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第2期17-21,共5页
Journal of Chongqing Normal University:Natural Science
基金
重庆市教委科学技术研究项目(No.KJ090827)
重庆市科技创新能力建设项目(No.2010CA1010)
重庆师范大学重点科研项目(No.08XLB001)
关键词
河川沙塘鳢
肝脏
血清
卵巢
卵黄蛋白原
Odontobutis potamophila, liver
serum
ovary
vitellogenin
