摘要
以石蒜为试材,研究了不同激素组合和外植体选择对石蒜愈伤组织诱导及植株再生的影响。结果表明:外植体选择是石蒜愈伤组织诱导的关键因素,3种外植体中,种子胚诱导愈伤组织的效果最好;在MS+2,4-D 1.0mg/L+6-BA 1.0mg/L培养基中,诱导愈伤率最高可达82.4%。并能较好地诱导愈伤组织发生不定芽,MS+NAA 0.1mg/L能较好地诱导愈伤组织发生根。石蒜再生植株进行移栽的最佳基质应为腐殖质和珍珠岩以1∶1的比例组成,其成活率为70%。
Taking Lycoruis radiate Herb. as material, the effect of some factors such as various hormone concentration, different parts of Lycoruis radiate Herb. on callus induction and plant regeneration were studied. The results showedthat the explants selection was the most significant factor of callus induction,and the seed embryos was the best explants for callus inductiorL The optimal mediums for the callus induction was MS＋2,4-D 1. 0 mg/L＋62BA 1. 0 mg/L and therate reached 82. 4%. In the subsequent callus further training, the optimal mediums for the callus induction was MS＋ 2,4-D 1. 0 mg/L＋6-BA 1. 0 mg/L,the optimal mediums for the callus induction was NAA 0. 1 mg/L. The best matrixfor Lycoris radiata regeneration plants was humus and perlite in the proportion of 1 ： 1 composition, with survival rate 70%.
出处
《北方园艺》
CAS
北大核心
2013年第6期93-96,共4页
Northern Horticulture
基金
江苏省教育厅资助项目(JH07-012)
镇江市科技局资助项目(NY2008034)
江苏省研究生培养创新工程资助项目(CXZZ12_0519)
关键词
石蒜
愈伤组织
植株再生
L ycoris radiate
callus
plant regeneration
