摘要
目的建立HPLC-ELSD法测定扶正散结胶囊中黄芪甲苷的含量。方法色谱柱Kromosil ODSC18(5μm,4.6 mm×250 mm),流动相为乙腈-水(32:68),流速:1.0 mL/min;柱温:室温。结果黄芪甲苷在1.0528～10.528μg范围内线性关系良好,平均加样回收率为98.74%,RSD为0.41%。结论所建立的本方法准确、快速、方便,可用于扶正散结胶囊的质量控制。
Establish an HPLC - ELSD method to measure the content of Astragaloside IV in reinforcing heslthy qi and relieving accumulation capsule. Methods The chromatographic column was Kromosil ODSCI8 （ 5μm, 4.6mm × 250mm ）, mobile phase was acetonitrile -water （ 32：68 ）, flow rate： 1. OmL＇ min; column temperature： room tempera- ture. Results the astragaloside IV in 1. 0528～10.528μg and the linear range, the average recovery was 98.74 %, RSD was 0.41%. Conclusion The method is accurate, fast, convenient, and can be used for the quality control of reinforcing beslthy qi and relieving accumulation capsule.
出处
《现代中医药》
CAS
2013年第2期86-88,共3页
Modern Chinese Medicine
基金
陕西省13115科技创新工程项目(2010ZDGC-22)
关键词
扶正散结胶囊
HPLC—ELSD
黄芪甲苷
Reinforcing heslthy qi and relieving accumulation
HPLC -ELSD
astragaloside
