摘要
目的:通过对内脏高敏性大鼠结肠MC密度、5-HT表达及二者相关性的影响,研讨痛泻要方对内脏高敏性大鼠结肠MC活化的干预作用。方法:采用乳鼠结肠刺激方法复制内脏高敏性大鼠IBS模型,以免疫组织化学方法和甲苯胺蓝改良法进行结肠双重复染法,观察痛泻要方对结肠组织MC形态学、密度的影响,及其与5-HT表达之间关系,并观察痛泻要方对其二者相关性的影响。结果:经乳鼠结肠刺激方法复制内脏高敏性模型后,结肠中MC颗粒增多,MC周边5-HT表达增强,二者有明显相关性,经痛泻要方干预后,结肠中MC形态改善、数量降低,5-HT表达降低,对二者相关性具有明显调节作用。结论:痛泻要方可改善内脏高敏性大鼠肠道MC活化程度,并抑制MC释放5-HT,从而降低5-HT表达。
Objective By studying on the effect on density of MC,the expression of 5-HT in intestinal and their relationship,to investigate the effect of Tongxie Yaofang on MC activation in visceral hypersensitivity rat colon.Methods：Establish IBS model of visceral hypersensitivity rats by milk mice colon irritation,to study the effect of Tongxie Yaofang on morphology and density of MC in colon,the expression of 5-HT,and the effect of Tongxie Yaofang on their correlation by double counterstaining method that immunohistochemical method and toluidine blue staining method.Results：After the model was established,particles in MC were increased,the expression of 5-HT around MC was enhanced,and there was an obvious correlation.After intervened by Tongxie Yaofang,the morphology of MC was improved,the quantity of MC was decreased and the expression of 5-HT was reduced.Its effect on their correlation had significantly changed.Conclusion： Tongxie Yaofang can improve degree of MC activation on visceral hypersensitivity rat in intestinal and inhibit MC release 5-HT to reduce the expression of 5-HT.
出处
《中医药学报》
CAS
2013年第1期82-85,共4页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省教育厅科研项目(No.12521502)
