摘要
目的 观察缺血预处理 (ischemicpreconditioning ,IPC)对缺血再灌注的大鼠肝脏的保护作用。 方法 建立大鼠肝脏缺血再灌注 (ischemiareperfusion ,I/R)损伤模型 ,选取肝脏损伤时的典型指标 :血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶 (LDH) ,肝组织中丙二醛 (MDA)、过氧化物歧化酶 (SOD)、组织中中性粒细胞 (PMNs)浸润量等 ,比较缺血预处理组与I/R组以及各预处理组间各种指标的含量变化情况 ,用统计学方法处理结果。结果 预处理组中ALT、AST、LDH、MDA、SOD、PMNs等指标较I/R组有明显变化。结论 各预处理组生化酶的含量和脂质过氧化物的含量减少 ,抗氧自由基能力增强 ,组织中PMNs浸润量降低提示缺血预处理对肝脏I/R损伤有显著的保护作用。尤其是一次缺血 10min再灌注 10min的预处理保护效果最佳。
Objective To observe the protective effects of preconditioning on liver ischemia reperfusion(I/R)injury in rats. Method Rat hepatic ischemia reperfusion model was used to evaluate the levels of plasma alanine aminotransferase(ALT), aspartate amino transferase(AST), lactate dehydrogenase(LDH), superoxide dismute(SOD), and the numbers of polymorphonuclear cells(PMNs). Results In preconditioning groups, leakage of enzymes and production of lipid peroxidation decreased, while the ability of anti-free radicals in plasma and liver increased. Infiltration of PMNs in the liver increased. Conclusion Preconditioning significantly protects liver from ischemia-reperfusion injury. Ten min ischemia followed by 10 min reperfusion produces better protection on liver I/R injury. [WT5”HZ]
出处
《山西医科大学学报》
CAS
2000年第5期388-389,共2页
Journal of Shanxi Medical University
