摘要
目的:观察川芎定痛颗粒对小鼠耳廓微循环障碍及气滞血瘀模型大鼠血液流变学指标的影响。方法:72只KM小鼠随机分为空白对照组(A组)、模型组(B组)、复方丹参滴丸组(C组)、川芎定痛颗粒高剂量组(D组)、川芎定痛颗粒中剂量组(E组)、川芎定痛颗粒低剂量组(F组),C～F组分别按0.135,1.75,0.88,0.44 g.kg-1灌胃给药3 d后,以耳廓局部滴加1∶1 000盐酸肾上腺素致小鼠耳廓局部微循环障碍,用BI2000数字图像分析系统观察小鼠在造模后10,20,30 min的微血管流速、口径、毛细血管开放量等指标的变化;60只SD大鼠分组与给药剂量与小鼠相同,灌胃给药5 d后,以冰水浴联合皮下注射盐酸肾上腺素制备"气滞血瘀"大鼠模型,取股动静脉混合血测定血液流变学指标。结果:川芎定痛颗粒能显著加快耳廓微循环障碍小鼠造模10,20,30 min的微血管流速(P<0.05),扩大其微血管静脉口径及微动脉口径(P<0.05),并且还能增加其毛细血管开放量(P<0.05);还能显著降低气滞血瘀大鼠模型的全血黏度(P<0.05)、全血高切相对指数(P<0.05),并且有降低全血低切相对指数趋势的作用。结论:川芎定痛颗粒具有活血化瘀作用。
Objective： To observe the effect of Chuangxiong Dingtong grainules （CXDT） on promoting blood circulation to dissipate blood stasis. Method： Seventy-two Kuenming mice were randomly divided into sex groups： normal control group （A） , model group （B）, compound Danshen dripping pills group （C）, high-dose CXDT group （D）, middle-dose CXDT group （E）, low-dose CXDT group （F）. Mice in group C-F were ig medicine O. 135, 1.75, 0.88, 0.44 g .kg-I for 3 days. 1： 1 000 epinephrine hydrochloride was dropped to the auricle of mice to induce local microcirculation disturbance. BI-2000 medical image analysis system was used to observe the microcirculation of mice at 10, 20, 30 minutes after dropping epinephrine hydrochloride. Sixty SD rats were grouped and gavaged as Kuenming mice. The qi-stagnancy and blood stasis rat model were established by epinephrine hydrochloride subcutaneous injection and ice water immertion. The femoral arteriovenous mixed-blood was used to assay hemorheology. Result： CXDT could significantly accelerate the microvascular flow rate of auricle microcirculation, expand its microvascular vein caliber and micro-artery diameter （P 〈0. 05 ）, increase the amount of open capillaries （ P 〈 0.05 ）, significantly reduce the whole blood viscosity and the high shear blood relative index of blood stasis rat mode （P 〈0. 05）. On the other hand, CXDT could reduce the trend of the whole blood relative index. Conclusion： Chuanxiong Dingtong grainules has the effect of promoting blood circulation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第7期174-177,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技支撑计划立项项目(2009BAI84B00)
