摘要
目的探讨Bcl-2和PTEN在宫颈癌组织、宫颈上皮内瘤样病变(CIN)和正常宫颈组织中表达情况及意义。方法采用组织芯片技术和免疫组织化学S-P法检测50例宫颈癌、31例CIN和20例正常宫颈组织中PTEN和Bcl-2的表达情况。结果 Bcl-2和PTEN表达定位于细胞浆和细胞核,其在宫颈癌中阳性表达率分别为82%(41/50)和32%(16/50),在CIN中的表达率分别为77%(24/31)和52%(16/31),在正常宫颈组织中表达率分别为0(0/20)和80%(16/20);两者在宫颈癌的表达情况均与淋巴结转移、临床分期及组织学分化程度相关(P均<0.05);在宫颈癌组织中Bcl-2和PTEN表达呈负相关(P<0.05)。结论随着癌前病变的进展及宫颈癌临床分期升高、病理组织学分化程度降低和淋巴结转移,PTEN和Bcl-2表达增强,说明两者与宫颈癌的发生、发展和转移密切相关。
Objective It is to explore the expression and its significance of PTEN and Bcl - 2 in the normal cervical tissue, CIN and cervical carcinoma. Methods The expressions of PTEN and Bcl - 2 in 50 cases of cervical carcinoma, 31 cases of CIN and 20 cases of normal endometrial tissue were detected by immunohistochemistry S - P. Results The expressions of PTEN and Bcl -2 were located in cytoplasm and the nucleus, and the positive expression rates were 82% （41/50） and 32% （16/50） respectively in cervical carcinoma, 77% （24/31） and 52% （16/31） in CIN, and 0（0/20） and 80% （16/20） in normal cervical tissue. Both of their expressions were related with lymph node metastasis, clinical stage and pathological staging（P 〈0.05）. In cervical carcinoma, the expressions of PTEN and Bcl- 2 were negative correlated with each other （P 〈 0.05）. Conclusion The expressions of PTEN and Bcl - 2 are increased along with the development of precancerous lesions and cervical cancer clinical staging elevation, pathological tissue differentiation of lower and lymph node metastasis, which shows that they are related with the development and metastasis of endometrial carcinoma .
出处
《现代中西医结合杂志》
CAS
2013年第11期1148-1149,1208,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
