摘要
目的观察正常兔眼玻璃体腔注射替考拉宁后对房水中细胞因子的影响。方法 24只健康日本大耳白兔,体质量2.5～3.0 kg,雌雄各半,随机分为实验组(替考拉宁1.0 mg)、对照组(灭菌生理盐水),于两组兔右眼玻璃体腔分别注射1 g/100 mL替考拉宁0.1 mL和灭菌生理盐水0.1 mL。术前和术后1、2、3、7、14和28 d抽取房水并采用ELISA法测定房水中TGF-β_2、IFN-γ、IL-6和IL-8的含量。结果术前实验组和对照组房水中TGF-β_2含量分别为(403.86±2.93)pg/mL和(403.83±2.30)pg/mL,术后两组TGF-β_2含量均下降(P<0.05)。术前实验组房水中IFN-γ、IL-6、IL-8含量分别为(340.41±17.36)pg/mL、(46.40±1.87)pg/mL、(44.73±1.37)pg/mL,对照组分别为(340.10±13.78)pg/mL、(46.56±1.86)pg/mL和(44.59±0.52)pg/mL;术后两组房水中IFN-γ、IL-6、IL-8均上升。结论玻璃体腔注射替考拉宁(1.0 mg)对房水中TGF-β_2、IFN-γ、IL-6和IL-8含量变化无影响。玻璃体腔注射操作可引起房水TGF-β_2含量下降,IFN-γ、IL-6和IL-8含量升高。
Objective To investigate the influence of teicoplanin on the cytokines in normal rabbit aqueous humor after intravitreal injection of teicoplanin. Methods Twenty-four rabbits were randomly assigned to two groups： the experimental group （teicoplanin 1.0 mg, 0.1 mL） and control group （normal saline 0.1 mL）, 12 rabbits per goup. The body weight of the animal was 2.5 to 3.0 kg. Teicoplanin or normal saline was injected into the vitreous body of the right eyes. The aqueous humor （0.1 mL） was extracted from every rabbit before operation and 1 d, 2 d, 3 d, 14 d, 28 d after operation. The concentration of TGF-β2, FN-y, 1L-6, and IL-8 were determined by enzyme linked immunosorbent assay. Results The concentration of TGF-β2 in aqueous humor was 403.86 ±2.93 pg/mL in the experimental group and 403.83 ± 2.30 pg/mL in control group before op eration. Its level began to reduce significantly after operation （P〈0.05）. The levels of IFN-y, IL-6, and IL-8 in aqueous hu mor was 340.41 ± 17.36 pg/mL, 46.40 ± 1.87 pg/mL, and 44.73 ± 1.37 pg/mL respectively in the experimental group be- fore operation, and increased after operation. The levels of IFN-7, IL-6, and 11. 8 in aqueous humor was 340. l0 ± 13.78 pg/ mL, 46.56 ±1.86 pg/mL, and 44.59 ±0.52 pg/mL respectively in control group before operation, and increased after operation. Conclusions Intravitreal injection of teicoplanin 1.0 mg did not influence the concentration of TGF-β2, IFN-7, IL-6 or IL- 8 in aqueous humor. However, intravitreal injection procedure may decrease the concentration of TGF-β2 and increase the con- centration of IFN-y, IL-6 and IL-8 in aqueous humor.
出处
《中国感染与化疗杂志》
CAS
北大核心
2013年第2期128-131,共4页
Chinese Journal of Infection and Chemotherapy
基金
河南省医学科技攻关项目(200904052)
关键词
替考拉宁
房水
细胞因子
teicoplanin aqueous humor cytokine
