摘要
对来自新疆地区艾丁湖、艾比湖和玛那斯盐湖等盐湖的２８株中度嗜盐菌与９株相关的参比菌株， 进行了１６Ｓ ｒＤＮＡ ＰＣＲ－ＲＦＬＰ分析，这些菌株是革兰氏阴性杆菌，能在０～２５％ＮａＣｌ中生长。在实验中， 选用４种限制性内切酶ＡｌｕＩ、ＨｉｎｆＩ、ＲｓａＩ和ＨａｅⅢ，将供试菌株的１６Ｓ ｒＤＮＡ的ＰＣＲ产物进行酶切，用３％ 的琼脂糖电泳分析酶切产物。结果表明，在７４％的相似性水平上分为３群。群Ⅰ包括新分离的菌株ＣＩ和 参比菌株死海色盐杆菌（Ｃｈｒｏｍｏｈａｌｏｂａｃｔｅｒ ｍａｒｉｓｍｏｒｔｕｉ）和Ｎｅｓｔｅｒｅｎｋｏｎｉａ ｈａｌｏｂｉａ；群Ⅱ包括伸长盐单胞（Ｈａｌｏｍｏｎａｓ ｅｌｏｎｇａｔａ）在内的７株参比菌株和８株新分离的菌株;群Ⅲ包括１９林新分离的菌株。
Twenty-eight moderately halophilic bacteria isolated from salt lakes in Xinjiang Regions were analyzed by 16S rDNA PCR-RFLP in comparison with nine reference strains of related species. The 16S rRNA PCR products of the tested stains were digested by four restriction enzymes (AluI, HinfI, RsaI and HaeⅢ). The results showed that all tested strains were clustered into three groups at 74% similarity level. Group I included 2 reference strains Chromohalobacter marismortui, Nesterenkonia halobia and new isolate CI. Group Ⅱ included seven type strains belonging to Halomonas and eight new isolates. Group Ⅲ included ninteen new isolates.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期327-330,共4页
Microbiology China
基金
国家自然科学基金资助项目
